Characterization of local immune responses associated with the parasitic resistance phenotype in morada nova lambs

• Grant number: 17/00373-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2017
• End date: March 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Ana Carolina de Souza Chagas
• Grantee: João Henrique Barbosa Toscano
• Host Institution: Embrapa Pecuária Sudeste. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil

Abstract

Brazil has great diversity of exotic sheep breeds and their crosses, as well as adapted breeds, like Morada Nova. However, gastrointestinal nematodes (GIN) are a major constrain in these production systems, specially Haemonchus contortus, prevalent species in the country. Morada Nova sheep, very adapted to tropical climate, are quite resistant to verminosis. Thus, the present study aims to characterize the parasitic resistance phenotype in lambs from the CPPSE Morada Nova flock, according to fecal egg counts (FEC), hematocrit and FAMACHA score. After two artificial infections with H. contortus L3, 20 animals (10 most resistant and 10 most susceptible) will be slaughtered for avaliation of the local immune response inducted by the parasite, by means of: (1) quantification of the expression immune response related genes (innate, pro-inflammatory, anti-inflammatory, regulatory T, humoral, Th1 profile and Th2 profile responses and complement system activation) in abomasum (fundic and pyloric regions) and abomasal lymph nodes, using RT-qPCR; (2) determination of IgG and IgA levels in abomasal mucus and (3) eosinophils and mast cell count in abomasal mucosa and submucosa. The identification of cytokines, cells and/or other mediators directly related to nematode resistance phenotype can be used to the improvement of the immune-prophylactic control of GIN infections. In the future, it would be possible to get mating generations, with homogeneous populations in relation to its composition, making it possible to offer resistant animals to producers, encouraging the use of Morada Nova sheep for meat production. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HAEHLING, MAREI B.; CRUVINEL, GIOVANNA G.; TOSCANO, JOAO H. B.; GIRALDELO, LUCIANA A.; SANTOS, ISABELLA B.; ESTEVES, SERGIO N.; BENAVIDES, V, MAGDA; BARIONI JUNIOR, WALDOMIRO; NICIURA, SIMONE C. M.; CHAGAS, ANA CAROLINA S.. Four single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep. Veterinary Parasitology, v. 279, MAR 2020. (17/24289-0, 17/01626-1, 17/00373-2)

OKINO, CINTIA HIROMI; MEO NICIURA, SIMONE CRISTINA; BARBOSA TOSCANO, JOAO HENRIQUE; ESTEVES, SERGIO NOVITA; DOS SANTOS, ISABELLA BARBOSA; VON HAEHLING, MAREI BORSCH; FIGUEIREDO, AMANDA; DE SENA OLIVEIRA, MARCIA CRISTINA; DE SOUZA CHAGAS, ANA CAROLINA. Ovine beta-globin gene: A new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infection. Veterinary Parasitology, v. 294, JUN 2021. (19/02929-3, 18/16070-1, 17/00373-2)

BARBOSA TOSCANO, JOAO HENRIQUE; LOPES, LOUYSE GABRIELLI; GIRALDELO, LUCIANA APARECIDA; DA SILVA, MATHEUS HENRIQUE; OKINO, CINTIA HIROMI; DE SOUZA CHAGAS, ANA CAROLINA. Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus. MOLECULAR BIOLOGY REPORTS, v. 45, n. 5, p. 1253-1262, OCT 2018. (17/00373-2, 17/24289-0, 17/01626-1)

OKINO, CINTIA HIROMI; FRIZONI CAMINOTTO, LARA CRISTINA; IKEDA KAPRITCHKOFF, RAFAELA TAMI; MEO NICIURA, SIMONE CRISTINA; ESTEVES, SERGIO NOVITA; DOS SANTOS, ISABELLA BARBOSA; BARBOSA TOSCANO, JOAO HENRIQUE; OLIVEIRA, M. C. S.; CHAGAS, A. C. S.. Comparison of ovine beta-globin haplotype sequences and a new multiplex PCR for identification. Veterinary Parasitology, v. 300, DEC 2021. (19/02929-3, 18/16070-1, 17/00373-2)

BARBOSA TOSCANO, JOAO HENRIQUE; DOS SANTOS, ISABELLA BARBOSA; VON HAEHLING, MAREI BORSCH; GIRALDELO, LUCIANA APARECIDA; LOPES, LOUYSE GABRIELLI; DA SILVA, MATHEUS HENRIQUE; FIGUEIREDO, AMANDA; ESTEVES, SERGIO NOVITA; DE SOUZA CHAGAS, ANA CAROLINA. Morada Nova sheep breed: Resistant or resilient to Haemonchus contortus infection?. Veterinary Parasitology, v. 276, p. 9-pg., 2019-01-01. (17/00373-2, 17/24289-0, 17/01626-1)

HAEHLING, MAREI B.; TOSCANO, JOAO HENRIQUE B.; GIRALDELO, LUCIANA A.; SANTOS, ISABELLA B.; ESTEVES, SERGIO N.; BENAVIDES, V, MAGDA; THOLON, PATRICIA; CHAGAS, ANA CAROLINA. S.. Is selection for resistance and resilience to Haemonchus contortus possible in Morada Nova sheep?. Small Ruminant Research, v. 201, AUG 2021. (17/00373-2, 17/01626-1, 17/24289-0)

NOGUEIRA, JEANE A. P.; FIGUEIREDO, AMANDA; DUARTE, JONATAS L.; DE ALMEIDA, FERNANDA B.; SANTOS, MARCELO G.; NASCIMENTO, LEONOR M.; FERNANDES, CAIO P.; MOURAO, SAMANTA C.; TOSCANO, JOAO HENRIQUE B.; ROCHA, LEANDRO M.; et al. Repellency effect of Pilocarpus spicatus A. St.-Hil essential oil and nanoemulsion against Rhipicephalus microplus larvae. Experimental Parasitology, v. 215, AUG 2020. (17/00373-2, 17/13249-8)

BARBOSA TOSCANO, JOAO HENRIQUE; OKINO, CINTIA HIROMI; BARBOSA DOS SANTOS, ISABELLA; GIRALDELO, LUCIANA APARECIDA; BORSCH VON HAEHLING, MAREI; NOVITA ESTEVES, SERGIO; BASSETTO, CESAR CRISTIANO; TALAMINI DO AMARANTE, ALESSANDRO FRANCISCO; DE SOUZA CHAGAS, ANA CAROLINA. Local and systemic immune mediators of Morada Nova lambs with divergentHaemonchus contortusresistance phenotypes. PARASITE IMMUNOLOGY, v. 42, n. 12, DEC 2020. (17/01626-1, 17/24289-0, 17/00373-2)

BARBOSA TOSCANO, JOAO HENRIQUE; OKINO, CINTIA HIROMI; DOS SANTOS, ISABELLA BARBOSA; GIRALDELO, LUCIANA APARECIDA; VON HAEHLING, MAREI BORSCH; ESTEVES, SERGIO NOVITA; DE SOUZA CHAGAS, ANA CAROLINA. Innate Immune Responses Associated with Resistance against Haemonchus contortus in Morada Nova Sheep. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2019, NOV 11 2019. (17/24289-0, 17/01626-1, 17/00373-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TOSCANO, João Henrique Barbosa. Characterization of local immune responses associated with the parasitic resistance phenotype in Morada Nova lambs. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.