Target selective treatment based on liveweight gain as nematode control strategy in Morada Nova lamb

• Grant number: 19/02929-3
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2020
• End date: April 30, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Ana Carolina de Souza Chagas
• Grantee: Ana Carolina de Souza Chagas
• Host Institution: Embrapa Pecuária Sudeste. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). São Carlos , SP, Brazil
• City of the host institution: São Carlos
• Associated researchers: Alessandro Francisco Talamini Do Amarante ; Alessandro Pelegrine Minho ; Cintia Hiromi Okino ; Fernanda de Freitas Anibal ; Magda Vieira Benavides ; Patricia Tholon ; Sergio Novita Esteves ; Simone Cristina Méo Niciura ; Waldomiro Barioni Júnior

Abstract

The research team of the present proposal carried out the FAPESP project "Genetic characterization and immune responses associated with the phenotype of parasitic resistance in the Morada Nova sheep flock" (2017/01626-1). It is now expected to advance in the knowledge generated by the evaluation of the 287 lambs and 123 matrices of the aforementioned project so that applicable technologies can be made available for the selective control of gastrointestinal nematodes (GIN) of small ruminants using as model the Morada Nova breed. It is intended to measure the impact of infection by GIN on the growth curve of the animals, in order to develop a model of deworming of the animals by means of selective treatment (TST), using the daily weight gain as indicator (Happy FactorTM). This strategy seems to be more adequate than the egg count per gram of feces (EPG), or even the FAMACHA© method, since Morada Nova lambs with high parasitic load remained with elevated hematocrit. Thus, in the future, the Happy FactorTM could be evaluated, because by means of this practice we can detect the most infected animals, optimize their weight gain and reduce the contamination of pasture by GIN, maintaining representative refuge and minimizing the selection of alleles of resistance. In addition, we intend to identify molecular markers associated with parasite resistance after genotyping of sheep on high density chip (GWAS), as well as to evaluate the main differentially expressed genes (RNAseq) between parasite infection extremes, to identify and select resistant animals. The future validation and availability of a TST would reduce costs with anthelmintics, drug residues in meat, dissemination of genes that confer resistance to anthelmintics, with consequent increase in their time of use. Thus, through the provision of practical and reliable parasitic control tools, the Morada Nova production in the state of São Paulo would be encouraged. (AU)

Scientific publications

(10)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

OKINO, CINTIA HIROMI; NICIURA, SIMONE CRISTINA MEO; GIARETTA, PAULA ROBERTA; MELITO, GLAUCIA ROBERTA; KAPRITCHKOFF, RAFAELA TAMI IKEDA; DOS SANTOS, ISABELLA BARBOSA; RECH, RAQUEL RUBIA; MINHO, ALESSANDRO PELEGRINE; ESTEVES, SERGIO NOVITA; CHAGAS, ANA CAROLINA DE SOUZA. Enhanced mucosal response in sheep harbouring beta(A) beta-globin against haemonchosis. PARASITE IMMUNOLOGY, v. N/A, p. 12-pg., 2023-07-03. (22/00776-8, 21/02535-5, 18/16070-1, 19/02929-3)

DE SOUZA CHAGAS, ANA CAROLINA; TUPY, OSCAR; DOS SANTOS, ISABELLA BARBOSA; ESTEVES, SERGIO NOVITA. Economic impact of gastrointestinal nematodes in Morada Nova sheep in Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 31, n. 3, p. 10-pg., 2022-01-01. (19/02929-3, 21/02535-5, 18/16070-1)

NICIURA, SIMONE CRISTINA MEO; BENAVIDES, MAGDA VIEIRA; OKINO, CINTIA HIROMI; IBELLI, ADRIANA MERCIA GUARATINI; MINHO, ALESSANDRO PELEGRINE; ESTEVES, SERGIO NOVITA; CHAGAS, ANA CAROLINA DE SOUZA. Genome-Wide Association Study for Haemonchus contortus Resistance in Morada Nova Sheep. PATHOGENS, v. 11, n. 8, p. 12-pg., 2022-08-01. (19/02967-2, 17/01626-1, 19/02929-3)

ANA CAROLINA DE SOUZA CHAGAS; OSCAR TUPY; ISABELLA BARBOSA DOS SANTOS; SÉRGIO NOVITA ESTEVES. Impacto econômico de nematódeos gastrintestinais em ovinos Morada Nova no Brasil. Revista Brasileira de Parasitologia Veterinária, v. 31, n. 3, 2022-08-19. (19/02929-3, 18/16070-1)

OKINO, CINTIA HIROMI; FRIZONI CAMINOTTO, LARA CRISTINA; IKEDA KAPRITCHKOFF, RAFAELA TAMI; MEO NICIURA, SIMONE CRISTINA; ESTEVES, SERGIO NOVITA; DOS SANTOS, ISABELLA BARBOSA; BARBOSA TOSCANO, JOAO HENRIQUE; OLIVEIRA, M. C. S.; CHAGAS, A. C. S.. Comparison of ovine beta-globin haplotype sequences and a new multiplex PCR for identification. Veterinary Parasitology, v. 300, DEC 2021. (19/02929-3, 18/16070-1, 17/00373-2)

OKINO, CINTIA HIROMI; MEO NICIURA, SIMONE CRISTINA; BARBOSA TOSCANO, JOAO HENRIQUE; ESTEVES, SERGIO NOVITA; DOS SANTOS, ISABELLA BARBOSA; VON HAEHLING, MAREI BORSCH; FIGUEIREDO, AMANDA; DE SENA OLIVEIRA, MARCIA CRISTINA; DE SOUZA CHAGAS, ANA CAROLINA. Ovine beta-globin gene: A new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infection. Veterinary Parasitology, v. 294, JUN 2021. (19/02929-3, 18/16070-1, 17/00373-2)

SANTOS, ISABELLA B.; FERREIRA, AVELARDO U. C.; RABELO, MARCIO D.; ANHOLETO, LUIS ADRIANO; SOUSA, GUSTAVO A.; GAINZA, YOUSMEL A.; FIGUEIREDO, AMANDA; ESTEVES, SERGIO N.; CHAGAS, ANA CAROLINA S.. Portable near-infrared spectroscopy: A rapid and accurate blood test for diagnosis of Haemonchus contortus infection and for targeted selective treatment of sheep. International Journal for Parasitology, v. 53, n. 2, p. 9-pg., 2023-02-17. (17/13249-8, 16/07132-8, 18/16070-1, 19/02929-3)

DOS SANTOS, ISABELLA BARBOSA; ANHOLETO, LUIS ADRIANO; DE SOUSA, GUSTAVO AVELAR; NUCCI, ALESSANDRA DA SILVA; GAINZA, YOUSMEL ALEMAN; FIGUEIREDO, AMANDA; LIMA DOS SANTOS, LEONARDO APARECIDO; MINHO, ALESSANDRO PELEGRINE; BARIONI-JUNIOR, WALDOMIRO; ESTEVES, SERGIO NOVITA; et al. Investigating the benefits of targeted selective treatment according to average daily weight gain against gastrointestinal nematodes in Morada Nova lambs. Parasitology Research, v. N/A, p. 12-pg., 2022-05-28. (19/02929-3, 18/16070-1)

NICIURA, SIMONE CRISTINA MEO; CARDOSO, TAINA FIGUEIREDO; IBELLI, ADRIANA MERCIA GUARATINI; OKINO, CINTIA HIROMI; ANDRADE, BRUNO GABRIEL; BENAVIDES, MAGDA VIEIRA; CHAGAS, ANA CAROLINA DE SOUZA; ESTEVES, SERGIO NOVITA; MINHO, ALESSANDRO PELEGRINE; REGITANO, LUCIANA CORREIA DE ALMEIDA; et al. Multi-omics data elucidate parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep. PARASITES & VECTORS, v. 17, n. 1, p. 19-pg., 2024-03-01. (21/02535-5, 19/02929-3, 21/11842-9, 22/06281-0, 17/01626-1)

BASSETTO, CESAR C.; ALBUQUERQUE, ANA CLAUDIA A.; LINS, JOSE GABRIEL G.; MARINHO-SILVA, NAIARA M.; CHOCOBAR, MARIANNA L. E.; BELLO, HORNBLENDA J. S.; MENA, MATEUS O.; NICIURA, SIMONE C. M.; AMARANTE, ALESSANDRO F. T.; CHAGAS, ANA CAROLINA S.. Revisiting anthelmintic resistance in sheep flocks from Sao Paulo State, Brazil. INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE, v. 24, p. 8-pg., 2024-03-05. (21/03479-1, 20/13972-4, 19/02929-3)