Application of MALDI Imaging (MSI) on non-nodulated and nodulated leaves of Psychotria and Hybanthus: Localization, analysis of cyclotides and its relationship with endosymbionts bacteria

• Grant number: 17/17098-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2017
• End date: April 30, 2022
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Eduardo Maffud Cilli
• Grantee: Meri Emili Pinto Nascimento
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery, AP.CEPID
• Associated scholarship(s): 19/04381-5 - The Last Advances on Solid-Phase Peptide Synthesis and NMR New Methodologies Applied to Cyclotides from Brazilian Plant Species, BE.EP.PD

Abstract

Some taxons of angiosperms biosynthesize peculiar substances known as cyclic peptides. The research on this class of natural products in Brazilian biodiversity species are relevant regarding the functional understanding of these molecules in plants where they occur. Being also relevant for the bioprospection of bioactive natural products, especially insecticidal, antimicrobial and antifungal. Published studies on the influence of peptides in signaling and plant defenses greatly highlight the role of these compounds in essential biological functions in species where they are produced. Peptides participate in plant signaling processes, regulate the closing of stomata, affect cell division, development, reproduction, nodulation and defense, and therefore becoming a fascinating research topic and still unexplored by research groups on natural products in Brazil. Aiming to study the identification, location and cyclotide function in nodulation, the leaf tissue of Hybanthus calceolaria, Psychotria vellosiana (non-nodulated) and Psychotria kirkii (nodulated) will be analyzed by an electron microscopy technique, thus allowing the location of nodes and endosymbiotic bacteria. Later on, imaging techniques for mass spectrometry (MALDI-MSI) and chemical identification by LC-MS / MS will be the cutting-edge tools used in this research project in order to map the production of these compounds in the tissues of the concerned species. Few studies are found in the literature involving MALDI-MSI approach on plant tissue and just only one is related to cyclotides. In Brazil there is no research involving this approach so far, becoming this subject a completely original research in the country. The Institute of Chemistry - UNESP, is now one of the institutions that are directing your research to this class of compounds and, therefore, being of great importance to the achievement of this project, within the trends in modern phytochemical approach. Due to several reports of biological activity shown by these compounds, it is also proposed in this project, the synthesis of isolated cyclotides, aiming further studies of antitumor activities and related to isolated endophytic bacteria.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOBEY, ANTONIO FERNANDEZ; FERREIRA PINTO, MERI EMILI; CILLI, EDUARDO MAFFUD; LOPES, NORBERTO PEPORINE; BOLZANI, VANDERLAN DA SILVA. A Cyclotide Isolated from Noisettia orchidiflora (Violaceae). Planta Medica, v. 84, n. 12-13, SI, p. 947+, AUG 2018. (17/17098-4, 13/07600-3, 14/50926-0)

PINTO, MERI EMILI F.; NAJAS, JHENNY Z. G.; MAGALHAES, LUMA G.; BOBEY, ANTONIO F.; MENDONCA, JACQUELINE N.; LOPES, NORBERTO P.; LEME, FLAVIA M.; TEIXEIRA, SIMONE P.; TROVO, MARCELO; ANDRICOPULO, ADRIANO D.; et al. Inhibition of Breast Cancer Cell Migration by Cyclotides Isolated from Pombalia calceolaria. Journal of Natural Products, v. 81, n. 5, p. 1203-1208, MAY 2018. (17/17098-4, 14/50265-3, 13/07600-3)

QUEIROZ, SUZANA APARECIDA S.; PINTO, MERI EMILI F.; BOBEY, ANTONIO F.; RUSSO, HELENA M.; BATISTA, ANDREA N. L.; BATISTA, JR., JOAO M.; CODO, ANA C.; MEDEIROS, ALEXANDRA I.; BOLZANI, VANDERLAN S.. Diterpenoids with inhibitory activity of nitrite production from Croton floribundus. Journal of Ethnopharmacology, v. 249, MAR 1 2020. (19/04381-5, 13/07600-3, 14/25222-9, 17/19870-6, 17/18807-9, 14/50926-0, 17/17098-4)

VALLI, MARILIA; RUSSO, HELENA MANNOCHIO; PILON, ALAN CESAR; FERREIRA PINTO, MERI EMILI; DIAS, NATHALIA B.; FREIRE, RAFAEL TEIXEIRA; CASTRO-GAMBOA, IAN; BOLZANI, VANDERLAN DA SILVA. Computational methods for NMR and MS for structure elucidation II: database resources and advanced methods. PHYSICAL SCIENCES REVIEWS, v. 4, n. 11, NOV 2019. (16/13292-8, 17/17098-4, 13/07600-3, 14/50926-0)

VALLI, MARILIA; RUSSO, HELENA MANNOCHIO; PILON, ALAN CESAR; FERREIRA PINTO, MERI EMILI; DIAS, NATHALIA B.; FREIRE, RAFAEL TEIXEIRA; CASTRO-GAMBOA, IAN; BOLZANI, VANDERLAN DA SILVA. Computational methods for NMR and MS for structure elucidation I: software for basic NMR. PHYSICAL SCIENCES REVIEWS, v. 4, n. 10, OCT 2019. (16/13292-8, 17/17098-4, 13/07600-3, 14/50926-0)

BOBEY, ANTONIO FERNANDEZ; FERREIRA PINTO, MERI EMILI; CILLI, EDUARDO MAFFUD; LOPES, NORBERTO PEPORINE; BOLZANI, VANDERLAN DA SILVA. A Cyclotide Isolated from Noisettia orchidiflora (Violaceae). Planta Medica, v. 84, n. 12-13, p. 7-pg., 2018-08-01. (14/50926-0, 17/17098-4, 13/07600-3)

SUELEM D. RAMALHO; MERI EMILI F. PINTO; RENATA K. ANDRICOPULO; PAULO R. S. SANCHES; EDILBERTO R. SILVEIRA; EDUARDO M. CILLI; ADRIANO D. ANDRICOPULO; VANDERLAN S. BOLZANI. Linear Peptide Analogues from Jatropha’s Orbitides Promote Migration of Human Neonatal Foreskin Fibroblasts in vitro and Collagen Deposition. Journal of the Brazilian Chemical Society, v. 30, n. 10, p. 2153-2161, 2019-10-21. (17/17098-4, 15/09533-7, 13/07600-3)

RAMALHO, SUELEM D.; PINTO, MERI EMILI F.; ANDRICOPULO, RENATA K.; SANCHES, PAULO R. S.; SILVEIRA, EDILBERTO R.; CILLI, EDUARDO M.; ANDRICOPULO, ADRIANO D.; BOLZANI, VANDERLAN S.. Linear Peptide Analogues from Jatropha's Orbitides Promote Migration of Human Neonatal Foreskin Fibroblasts in vitro and Collagen Deposition. Journal of the Brazilian Chemical Society, v. 30, n. 10, SI, p. 2153-2161, OCT 2019. (15/09533-7, 17/17098-4, 13/07600-3)

PINTO, MERI EMILI F.; CHAN, LAI YUE; KOEHBACH, JOHANNES; DEVI, SEEMA; GRUNDEMANN, CARSTEN; GRUBER, CHRISTIAN W.; GOMES, MARIO; BOLZANI, VANDERLAN S.; CILLI, EDUARDO MAFFUD; CRAIK, DAVID J.. Cyclotides from Brazilian Palicourea sessilis and Their Effects on Human Lymphocytes. Journal of Natural Products, v. 84, n. 1, p. 81-90, JAN 22 2021. (19/04381-5, 17/17098-4, 13/07600-3)

FERNANDEZ-BOBEY, ANTONIO; FERREIRA PINTO, MERI EMILI; DE ALMEIDA, LARISSA COSTA; DE SOUZA, BIBIANA MONSON; DIAS, NATHALIA BAPTISTA; DE PAULA-SOUZA, JULIANA; CILLI, EDUARDO MAFFUD; LOPES, NORBERTO PEPORINE; COSTA-LOTUFO, LETICIA VERAS; PALMA, MARIO SERGIO; et al. Cytotoxic Cyclotides from Anchietea pyrifolia, a South American Plant Species. Journal of Natural Products, v. 85, n. 9, p. 8-pg., 2022-08-31. (14/50926-0, 19/04381-5, 20/02207-5, 14/25222-9, 13/07600-3, 19/26550-3, 17/17098-4, 16/16212-5)

RAMALHO, SUELEM D.; PINTO, MERI EMILI F.; ANDRICOPULO, RENATA K.; SANCHES, PAULO R. S.; SILVEIRA, EDILBERTO R.; CILLI, EDUARDO M.; ANDRICOPULO, ADRIANO D.; BOLZANI, VANDERLAN S.. Linear Peptide Analogues from Jatropha's Orbitides Promote Migration of Human Neonatal Foreskin Fibroblasts in vitro and Collagen Deposition. Journal of the Brazilian Chemical Society, v. 30, n. 10, p. 9-pg., 2019-10-01. (13/07600-3, 17/17098-4, 15/09533-7)