Chemical characterization of pigments of biotechnological interest from bacteria isolated from Antarctica

• Grant number: 17/21790-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): January 17, 2018
• Effective date (End): April 16, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Valeria Maia Merzel
• Grantee: Tiago Rodrigues e Silva
• Supervisor: Ramon Canela-Garayoa
• Host Institution: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA). Universidade Estadual de Campinas (UNICAMP). Paulínia , SP, Brazil
• Research place: Universidad de Lleida (UdL), Spain
• Associated to the scholarship: 14/17936-1 - Microbial prospecting for antibiotics and pigments from bacteria isolated from Antarctica., BP.DR

Abstract

This study aims to develop and improve skills related to pigments identification. Recently, four pigmented bacteria from the Antarctic continent were able to withstand UV-B and UV-C radiation - Arthrobacter agilis 50-cyt, Celullophaga fucicola 416, Zobellia laminarie 465 and Arthrobacter alpinus 366. The pigments were extracted and characterized by the UV spectrum absorbance. In addition, they were tested for antioxidant capacity, UV light stability and phototoxicity profile against murine fibroblast lines to verify the potential use as sunscreens. The results until the moment showed that all the pigments presented a very high antioxidant action; good stability on exposure to UV light; and a fraction of the A. agilis pigment did not express phototoxic action. To finish the study it is necessary to identify these pigments and a partnership was signed with the group of Dr. Ramon Canela Garayoa of the University of Lleida, specialists in the identification of carotenoids.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, TIAGO R.; CANELA-GARAYOA, RAMON; ERAS, JORDI; RODRIGUES, MARILI V. N.; DOS SANTOS, FABIO N.; EBERLIN, MARCOS N.; NERI-NUMA, IRAMAIA A.; PASTORE, GLAUCIA M.; TAVARES, RENATA S. N.; DEBONSI, HOSANA M.; et al. Pigments in an iridescent bacterium, Cellulophaga fucicola, isolated from Antarctica. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, v. 112, n. 3, p. 479-490, MAR 2019. (16/05640-6, 17/21790-0, 14/17936-1)

SANTA-CRUZ VASQUEZ, YESENIA MELISSA; GOMES, MILENE BARBOSA; RODRIGUES E SILVA, TIAGO; FERNANDES DUARTE, ALYSSON WAGNER; ROSA, LUIZ HENRIQUE; DE OLIVEIRA, VALERIA MAIA. Cold-adapted chitinases from Antarctic bacteria: Taxonomic assessment and enzyme production optimization. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 34, JUL 2021. (14/17936-1, 17/21790-0, 16/05640-6)

SILVA, TIAGO R.; TAVARES, RENATA S. N.; CANELA-GARAYOA, RAMON; ERAS, JORDI; RODRIGUES, MARILI V. N.; NERI-NUMA, IRAMAIA A.; PASTORE, GLAUCIA M.; ROSA, LUIZ H.; SCHULTZ, JOSE A. A.; DEBONSI, HOSANA M.; et al. Chemical Characterization and Biotechnological Applicability of Pigments Isolated from Antarctic Bacteria. MARINE BIOTECHNOLOGY, v. 21, n. 3, p. 416-429, JUN 2019. (16/05640-6, 17/21790-0, 14/17936-1)