Adsorption of human IgG on polyacrylamide/alginate monolithic cryogel-Immobilized o-phosphotyrosine (OPT)

• Grant number: 17/21751-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2018
• End date: July 31, 2019
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Sonia Maria Alves Bueno
• Grantee: Camila Marcuz
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Antibodies (Immunoglobulins) are glicoproteins employed in the analytical, therapeutic, diagnostic, biosensor, and proteomic areas. Purification of IgG molecules is critical, since homogenous antibody preparations are usually required for these applications. Also, the need for large doses of antibodies for therapeutic treatments led researchers develop new chromatographic techniques for antibodies separation. In the last years, one the developed in this area is the monolithic beds as stationary phase in chromatography. For their macroporous structure, the monolithic beds have suitable properties for purification of macromolecules, like macroporous structure, low resistance to flow through the column and mass transfer governed mainly by convection. This project aims to develop an adsorbent based on acrylamide, allyl diglidyl ether, and alginate, prepared by the technique of cryo-polymerization, to be used as a monolithic stationary phase for the adsorption of human IgG. The pseudobiospecific ligand ortho-phosphotyrosine (OPT) will be immobilized in this cryogel, since our research group demonstrated that this ligand selectively adsorbed IgG from human plasma. The cryogels prepared based on polyacrylamide and alginate will be characterized as porosity, morphology, and water absorption. The adsorption capacity of IgG from human serum will be evaluated and the portion of IgG (Fab or Fc) adsorbed on the immobilized ligand will be evaluated. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARCUZ, CAMILA; MOURAO, CECILIA ALVES; HAUPT, KARSTEN; ALVES BUENO, SONIA MARIA. Performance of phospho-L-tyrosine immobilized onto alginate/ polyacrylamide-based cryogels: Effect of ligand coupling on human IgG adsorption and Fab fragments separation. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1165, FEB 15 2021. (17/21751-5, 16/10855-1)

MOURAO, CECILIA ALVES; MARCUZ, CAMILA; HAUPT, KARSTEN; ALVES BUENO, SONIA MARIA. Polyacrylamide-alginate (PAAm-Alg) and phospho-L-tyrosine-linked PAAm-Alg monolithic cryogels: Purification of IgG from human serum. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1129, OCT 15 2019. (16/10855-1, 17/21751-5)

MARCUZ, CAMILA; MOURAO, CECILIA ALVES; HAUPT, KARSTEN; ALVES BUENO, SONIA MARIA. Performance of phospho-L-tyrosine immobilized onto alginate/polyacrylamide-based cryogels: Effect of ligand coupling on human IgG adsorption and Fab fragments separation. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1165, p. 12-pg., 2021-01-21. (17/21751-5, 16/10855-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARCUZ, Camila. Synthesis and evaluation of monolithic cryogel containing reactive group and o-phospho-l-tirosine for adsorption of IgG and its fragments. 2019. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química Campinas, SP.