Development, validation and reliability of a general and facial sedation scale in horses

• Grant number: 17/16208-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2017
• End date: May 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Stelio Pacca Loureiro Luna
• Grantee: Alice Rodrigues de Oliveira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Sedation in horses is a target of studies that evaluate the sedative effects of drugs in experimental and clinical situations. There are different forms already described to estimate the response of the animals, however without standartization and validation, which makes reproducibility and comparison of scientific results difficult. To fill this gap, we aim to develop a general and facial sedation scale based on previous descriptions and validate them for research applicability. Linked to the thematic project of process number 2017/12825-0, in the present study, 7 healthy horses will be submitted to different sedation protocols with detomidine isolated or associated to methadone and a placebo group, all in continuous infusion for a period of 2 hours and evaluated for a period of 4 hours. Videocameras will be installed to footage analysis by one observer and three other covered. Photographs of the facial expression of the animals will be taken at each moment, followed by the realization of stimulli by a second resercher, whose answers will be evaluated by the main investigator. Inter and intra-observers and test reterst reliability will be performed; the content analysis throught the importance of each item related to the scale as a whole; Internal consistency of the variables; the validity of the construct by multivariate analysis; the validity of the criterion through the Spearman correlation with the gold standard (Analogue Visual Scale); the item-total correlation by the Spearman correlation between each item and the sum of the items of the scale, the reliability intra-observer by the kappa coefficient and the inter-observer by the intra-class correlation coefficient. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ALICE RODRIGUES; GOZALO-MARCILLA, MIGUEL; RINGER, SIMONE KATJA; SCHAUVLIEGE, STIJN; FONSECA, MARIANA WERNECK; ESTEVES TRINDADE, PEDRO HENRIQUE; PROSPERO PUOLI FILHO, JOSE NICOLAU; LOUREIRO LUNA, STELIO PACCA. Development and validation of the facial scale (FaceSed) to evaluate sedation in horses. PLoS One, v. 16, n. 6, 2021. (17/01425-6, 17/12815-0, 17/16208-0)

OLIVEIRA, ALICE RODRIGUES DE; GOZALO-MARCILLA, MIGUEL; RINGER, SIMONE KATJA; SCHAUVLIEGE, STIJN; FONSECA, MARIANA WERNECK; TRINDADE, PEDRO HENRIQUE ESTEVES; PUOLI FILHO, JOSE NICOLAU PROSPERO; LUNA, STELIO PACCA LOUREIRO. Development, Validation, and Reliability of a Sedation Scale in Horses (EquiSed). FRONTIERS IN VETERINARY SCIENCE, v. 8, FEB 16 2021. (17/16208-0, 17/01425-6, 17/12815-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Alice Rodrigues de. Development, validation and reliability of a general and facial sedation scale in horses. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.