Shotgun proteomics for host-pathogen relationship investigation of leishmaniasis in vitro and in vivo

• Grant number: 17/22651-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: February 26, 2018
• End date: February 25, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Marcos Nogueira Eberlin
• Grantee: Fernanda Negrão Silva
• Supervisor: John Yates III
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: Scripps Research Institute, San Diego, United States
• Associated to the scholarship: 16/11517-2 - Cutaneous leishmaniasis biomarkers candidates search and identification through mass spectrometry, BP.DD

Abstract

Leishmaniases is group of diseases caused by more than 20 different species of Leishmania and causes a wide variety of clinical forms depending on the species. Proteomics have improved our understanding of the pathogenesis of Leishmaniasis considering a variety of aspects of parasite biology and host interactions. However, the route used by protozoa to eventually reach visceral organs and details about Leishmania-host interactions are still poorly understood. The chosen methodology for this project is the Multidimensional Protein Identification Technology (MudPIT), a state-of-the-art technique in proteomics, which allows detection of a huge number of proteins, even those present in low abundance. To our knowledge, this will be the first gel-free proteomics of murine cutaneous leishmaniasis lesions and of an in vitro model for infection comparing three different etiological agents (L. amazonensis, L. major and L. infantum) using J774 macrophages. Thus, we expect to unveil more information on Leishmania-host interaction opening the possibility to identify novel targets for vaccination and chemotherapeutic strategies against leishmaniasis. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NEGRAO, FERNANDA; DIEDRICH, JOLENE K.; GIORGIO, SELMA; EBERLIN, MARCOS N.; YATES, III, JOHN R.. Tandem Mass Tag Proteomic Analysis of in Vitro and in Vivo Models of Cutaneous Leishmaniasis Reveals Parasite-Specific and Nonspecific Modulation of Proteins in the Host. ACS INFECTIOUS DISEASES, v. 5, n. 12, SI, p. 2136-2147, DEC 2019. (15/23767-0, 17/22651-4)

NEGRAO, FERNANDA; DIEDRICH, JOLENE K.; GIORGIO, SELMA; EBERLIN, MARCOS N.; YATES, JOHN R., III. Tandem Mass Tag Proteomic Analysis of in Vitro and in Vivo Models of Cutaneous Leishmaniasis Reveals Parasite-Specific and Nonspecific Modulation of Proteins in the Host. ACS INFECTIOUS DISEASES, v. 5, n. 12, p. 23-pg., 2019-12-01. (15/23767-0, 17/22651-4)

NEGRAO, FERNANDA; GIORGIO, SELMA; EBERLIN, MARCOS NOGUEIRA; YATES, III, JOHN R.. Comparative Proteomic Analysis of Murine Cutaneous Lesions Induced by Leishmania amazonensis or Leishmania major. ACS INFECTIOUS DISEASES, v. 5, n. 8, p. 1295-1305, AUG 2019. (15/23767-0, 17/22651-4)

NEGRAO, FERNANDA; FERNANDEZ-COSTA, CAROLINA; ZORGI, NAHIARA; GIORGIO, SELMA; EBERLIN, MARCOS NOGUEIRA; YATES, III, JOHN R.. Label-Free Proteomic Analysis Reveals Parasite-Specific Protein Alterations in Macrophages Following Leishmania amazonensis, Leishmania major, or Leishmania infantum Infection. ACS INFECTIOUS DISEASES, v. 5, n. 6, p. 851-862, JUN 2019. (15/23767-0, 17/22651-4)