Resistance exercise training effect upon pancreatic beta cell in in-vitro and in-vivo type-1 diabetic models

• Grant number: 17/25771-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2018
• End date: January 31, 2019
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Antonio Carlos Boschiero
• Grantee: Jaqueline Fernandes de Barros
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 15/12611-0 - Molecular mechanisms involved in pancreatic beta cell disfunction and dead in diabetes mellitus: strategies for the inhibition of these processes and restoration of the insular mass, AP.TEM

Abstract

Resistance exercise training helps to control the glycemia in type-1 diabetic (T1-D) patients. However, mostly of studies on this matter, focuses on the involvement of skeletal muscle in this context. Whereas the impact of resistance exercises training upon pancreatic beta cell of diabetic animals was neglected. Here, we aim to verify if resistance exercise training promotes positive effects in pancreatic beta cell in both: in-vivo and in-vitro T1-D models. So, we will analyze the morphological aspects (number and mass of cells), and function (insulin secretion) in streptozotocin-induced T1-D mice. Also, we will verify if the responses of pancreatic beta cell to resistance exercise training are linked with a cross-talk mechanism between skeletal muscle and endocrine pancreas. For this purpose, we will treated the pancreatic beta cell line (INS-1E) with 10% of serum from trained control mice and then, expose this cell to a pro-inflammatory cytokines mix (in-vitro T1-D model). After we will analyze cell death, survive, and viability through specific assays. The possibility to find new molecules that are secreted by the skeletal muscle, during different types of exercise training, that benefits the pancreatic beta cells may have a significant potential in the diabetes treatment. (AU)