Effect of Trypanosoma cruzi cyclophilin 19 on mammalian cells

• Grant number: 17/19303-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2018
• End date: December 31, 2019
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Sergio Schenkman
• Grantee: Fernanda Midori Abukawa
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 15/22031-0 - Cell signaling in Trypanosoma during host-parasite interaction, AP.TEM

Abstract

Considering the similarity of Cyclophilin 19, T. cruzi with human Cyclophilin A, and the already consolidated literature regarding the role of CyPA in inflammatory processes and in various heart diseases, this project aims to verify if cyclophilin 19 is expressed in the forms of T. cruzi that are in contact with mammalian cells have some effect on cellular responses and thus determine whether it has a relationship with cardiac inflammation present in Chagas Disease. For this we intend to express in an inducible manner the CyP19 in T. cruzi in myoblasts and to analyze the phenotypes related to inflammatory responses and resistance to apoptosis in these cells (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, GREGORY PEDROSO; ABUKAWA, FERNANDA MIDORI; SOUZA-MELO, NORMANDA; ALCANTARA, LAURA MARIA; BITTENCOURT-CUNHA, PAULA; MORAES, CAROLINA BORSOI; JHA, BIJAY KUMAR; MCGWIRE, BRADFORD S.; MORETTI, NILMAR SILVIO; SCHENKMAN, SERGIO. Cyclophilin 19 secreted in the host cell cytosol by Trypanosoma cruzi promotes ROS production required for parasite growth. Cellular Microbiology, v. 23, n. 4, DEC 2020. (18/09948-0, 17/02416-0, 17/19303-4, 18/07766-2)