Expression of neuroactive substances in auditory pathway encephalic centers in aging

• Grant number: 17/09484-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2018
• End date: May 31, 2019
• Field of knowledge: Health Sciences - Speech Therapy
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Ana Claudia Figueiredo Frizzo
• Grantee: Graziela Ligia da Silva Santos
• Host Institution: Faculdade de Filosofia e Ciências (FFC). Universidade Estadual Paulista (UNESP). Campus de Marília. Marília , SP, Brazil

Abstract

During aging, in mammals, could occur presbycusis, characterized by loss of cells in the cochlea or changes in the central auditory system (SAC) function, which may lead to impairment of speech compression in the elderly. Calcium-binding proteins (CaBPs) are essential for processing at synaptic stations of the auditory pathway and changes in this expression during aging could be part of the mechanism involved in presbycusis. On the other hand, the hormone melatonin with its antioxidant action, could minimize the oxidative stress on aging, representing an otoprotective effect on the auditory system. However, it is not clear whether this hormone would act only peripherally or whether the SAC stations will be provided with melatonin receptors. Such investigations will contribute to the understanding of the mechanisms involved in presbycusis and a possible otoprotective action of melatonin in aging. Objective: To evaluate CaBPs, MT1 and MT2 receptors differences in the synaptic stations that of the SAC between young and elderly rats. Methods: Brain slices from young and elderly rats (Wistar) will be processed using immunohistochemistry to evaluate the expression of parvalbumin (PV), calbindin (CB), calretinin (CR) and melatonin receptors (MT1 and MT2). Quantification of the number of CaBP positive cells will be performed by stereology and receptor expression will be assessed by optical density. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Graziela Ligia da Silva. Expression of calcium-binding proteins and melatonin receptors in auditory pathway encephic centers in aging. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Filosofia e Ciências. Marília Marília.