Construction and characterization of adenoviral vectors encoding the canine cDNAs for p14Arf and IFN-beta

• Grant number: 17/25284-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: February 01, 2018
• End date: March 31, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Bryan Eric Strauss
• Grantee: Otavio Augusto Rodrigues
• Host Institution: Instituto do Câncer do Estado de São Paulo Octavio Frias de Oliveira (ICESP). São Paulo , SP, Brazil
• Associated research grant: 15/26580-9 - Cancer gene therapy: strategic positioning for translational studies, AP.TEM

Abstract

This sub-project (presented in the main proposal as project 10) describes the steps we will take to reach the proposal of a clinical gene therapy protocol for the treatment of canine Melanoma. We propose that immunotherapy with p14Arf and IFN-beta may be beneficial in the treatment of Melanoma. In preparation for pre-clinical trials of experimental gene therapy in humans, the treatment of Melanoma in dogs may reveal the proper functioning of our approach. The clinical aspects of human and canine Melanoma are similar and the treatment of dogs as demonstration of efficacy of new therapies is relevant. In the future, we hope to treat canine patients with Melanoma with our immunotherapy mediated by the adenoviral vector bearing p14Arf and IFN-beta. At the moment, we will start with the assembly and characterization of the vectors carrying the canine versions of p14Arf and IFN-beta cDNAs. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOMINGUES, ANA CAROLINA M.; DE OLIVEIRA, SORAIA B.; TESSAROLLO, NAYARA G.; LEPIQUE, ANA PAULA; RODRIGUES, OTAVIO; SHAROVA, TATYANA; LAWLESS, ALEIGHA; LI, DAVID; BASNET, MADAK; BOLAND, GENEVIEVE M.; et al. Use of patient-derived organotypic tumor spheroids for testing of viral vector gene therapy in combination with checkpoint blockade. MOLECULAR THERAPY ONCOLOGY, v. 33, n. 1, p. 12-pg., 2025-02-13. (17/25290-2, 18/25555-9, 17/25284-2, 22/15913-0, 15/26580-9, 22/04368-1)

TESSAROLLO, NAYARA GUSMAO; DOMINGUES, ANA CAROLINA M.; ANTUNES, FERNANDA; DOS SANTOS DA LUZ, JEAN CARLOS; RODRIGUES, OTAVIO AUGUSTO; DUTRA CERQUEIRA, OTTO LUIZ; STRAUSS, BRYAN E.. Nonreplicating Adenoviral Vectors: Improving Tropism and Delivery of Cancer Gene Therapy. CANCERS, v. 13, n. 8, APR 2021. (17/25290-2, 17/23068-0, 17/25284-2, 18/25555-9)

CERQUEIRA, OTTO LUIZ DUTRA; ANTUNES, FERNANDA; ASSIS, NADINE G.; CARDOSO, ELAINE C.; CLAVIJO-SALOMON, MARIA A.; DOMINGUES, ANA C.; TESSAROLLO, NAYARA G.; STRAUSS, BRYAN E.. Perspectives for Combining Viral Oncolysis With Additional Immunotherapies for the Treatment of Melanoma. FRONTIERS IN MOLECULAR BIOSCIENCES, v. 9, p. 23-pg., 2022-04-14. (15/26580-9, 17/25284-2, 19/03055-7, 18/04800-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RODRIGUES, Otavio Augusto. Canine model of melanoma immunotherapy using adenoviral vectors carrying p14Arf and interferon-beta cDNAs. 2023. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.