Study of the formation of the mitoribosome exit tunnel and impact in mitochondria translation

• Grant number: 18/03253-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2018
• End date: June 30, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Mario Henrique de Barros
• Grantee: Barbara Santos de Oliveira
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID

Abstract

Our lab aim is the study of mitochondria biogenesis in yeast. Here we propose to study the formation of the mitoribosome exit tunnel and the impact of protein extensions of ribosome proteins not present in other types of ribosomes, and therefore unique of the mitoribosome. After the generation of new alleles we will test them in the respective null mutants checking for respiratory and translational capacity. At the end we would like to obtain information about the formation of mitoribosome intermediates, which can provide new informations about the role of these unique extensions on mitoribosome assembly. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, BARBARA; ZENG, RUI; JORGE, SASA F.; FERREIRA-JUNIOR, JOSE RIBAMAR; BARRIENTOS, ANTONI; BARROS, MARIO H.. Functional analyses of mitoribosome 54S subunit devoid of mitochondria-specific protein sequences. YEAST, NOV 2021. (13/07937-8, 20/05812-7, 18/03253-0, 17/23921-5)