Treatment of synovitis in horses: effect of mesenchymal stem cells involved by microcapsules on the articular inflammatory mediators

• Grant number: 17/01369-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2018
• End date: June 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Ana Liz Garcia Alves
• Grantee: Gustavo dos Santos Rosa
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The high exigency on the performances of equine athletes often results in small focal articular injuries. Cumulatively, they can cause synovitis and consequential release of several inflammatory mediators within the articular microenvironment. Without correct treatment, the synovitis leads to various events, resulting in osteoarthritis development. Regenerative therapies, as the use of mesenchymal stem cells (MSC), have been largely applied aiming to stop the progression of this disease. Involving the MSC in hydrogel microcapsules is an encouraging method for articular inflammatory suppression and delivery of therapeutic agents. The aim of this study is to evaluate the behavior of allogeneic MSC derived from synovial membrane and involved by bioabsorbable microcapsules over the inflammatory mediators on the treatment of tibiotarsal synovitis in horses. Fifteen horses divided in three equal groups will be submitted to experimental tibio-tarsal synovitis. They will be treated with free MSCs, encapsulated MSCs or buffered saline. Harvests of synovial fluid will allow quantifying the inflammatory mediators, performing conventional laboratorial analysis and determining oxidative stress, aiming to compare the immunomodulatory effect between the different therapies. A higher immunomodulatory effect in the group treated with MSCs involved by microcapsules is expected. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, JAQUELINE BRANDAO; ROSA, GUSTAVO DOS SANTOS; ROSSI, MARIANA CORREA; STIEVANI, FERNANDA DE CASTRO; PFEIFER, JOAO PEDRO HUBBE; TERAMOTO KRIECK, ANDRE MASSAHIRO; DE CARVALHO BOVOLATO, ANA LIVIA; FONSECA-ALVES, CARLOS EDUARDO; AMIGO BORRAS, VICENTE; GARCIA ALVES, ANA LIZ. In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 9, AUG 27 2021. (17/01369-9, 17/12815-0, 17/14460-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROSA, Gustavo dos Santos. Synovitis in horses: effect of mesenchymal stem cells on the articular inflammation.. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.