Full text
| |
| Author(s): |
Gustavo dos Santos Rosa
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Botucatu. 2019-07-02. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu |
| Defense date: | 2019-06-25 |
| Advisor: | Ana Liz Garcia Alves |
| Abstract | |
The high requirement of the locomotor system of equine athletes often results in small joint injuries that can cause synovial inflammation and release of several inflammatory mediators in the articular microenvironment, leading to the development of osteoarthritis (OA). Mesenchymal stem cells (MSCs) are used in these cases to modify the progression of this disease. This study aimed to verify the behavior of allogeneic synovial membrane derived MSCs (MSCsm) against inflammatory events in acute equine synovitis. Synovitis was induced by the intra-articular (IA) administration of 0.5 ng of LPS and the subjects were treated 8 hours post-induction. The control group received 2 ml of PBS IA as treatment, while the treated group received 107 MSCsm IA. Physical exams, lameness evaluations, cytological analysis of synovial fluid and determination of synovial concentrations of TGF-β and PGE2 were performed. No differences were observed in lameness and physical exam parameters between the groups. Cytological analysis revealed a significant decrease in lymphocytes and macrophages in the treated group (P = 0.0059 and P = 0.0003, respectively), which also had lower levels of TGF-β (P = 0.0467) at 24 h. The other parameters did not present significant difference at any time. Our data suggest that acute inflammation initially caused inhibition of the immunomodulatory capacity of MSCs, which was resumed after 24h with the decline of joint inflammation, and that the interaction of MSCs with the inflammatory mediators present in the synovial fluid is directly related to the condition inflammatory at the time of application. To optimize the immunomodulatory potential of MSCs, the authors suggest the adoption of strategies to control local inflammation prior to its administration. (AU) | |
| FAPESP's process: | 17/01369-9 - Treatment of synovitis in horses: effect of mesenchymal stem cells involved by microcapsules on the articular inflammatory mediators |
| Grantee: | Gustavo dos Santos Rosa |
| Support Opportunities: | Scholarships in Brazil - Master |