| Grant number: | 17/13213-3 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | April 01, 2018 |
| End date: | June 30, 2020 |
| Field of knowledge: | Health Sciences - Medicine - Surgery |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Luís Otávio Zanatta Sarian |
| Grantee: | Francisco Claro de Oliveira Junior |
| Host Institution: | Centro de Atenção Integral à Saúde da Mulher (CAISM). Hospital da Mulher Professor Doutor José Aristodemo Pinotti. Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
Abstract An experimental model aiming to evaluate the microenvironment of breast cancer is very important for the preclinical analysis of risk factors and the behavior of this disease to possible treatments proposed in humans. The preclinical studies used so far for this purpose present serious methodological problems. They are based on models that use cancer-induced carcinogens that have a residual systemic effect or through the use of non-luminal human mammary tumors implanted in immunosuppressed murine. In the first case, we have contamination and permanence of the carcinogenic action in the animal. In the second case, it is used animals devoid of natural defense against cancer (because they are immunosuppressed); in addition to not being able to study in vivo the most prevalent tumors (luminal tumors, which represent more than 75% of cases of breast cancer in humans). Objective: Based on the researcher's experience in previous studies, which identified a possible breast cancer inducer to which humans are exposed and which has no residual effect, this project is proposed aiming to develop a preclinical model capable of adequately assessing the microenvironment of breast cancer. Subjects and methods: 60 Sprague-Dawley rats, randomly divided into 2 groups: group 1 - rats fed standard diet (Sham) and Group 2 - rats that will receive a cafeteria diet after 28 days of life. The rats will have the diet replaced for the regular diet for rodents after the formation of breast tumors. Samples of the six thoracic breasts, adipose tissue of omentum and subcutaneous from each rat will be analyzed after the death induction of animals. Animals will be monitored weekly for tumor size and death will be induced if tumor evolution is causing suffering for the animal. At 18 months of age (mean expected age of life of this animal under normal conditions), the study will be completed and tissue samples of the rats will be collected for analysis. (AU) | |
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