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Impact of teat channel occlusion on the metagenomic profile and its reflex in health of the bovine mammary gland

Grant number: 18/03685-8
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2018
Effective date (End): June 30, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Alice Maria Melville Paiva Della Libera
Grantee:José Augusto Ferronatto
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In recent years, with the advancement of molecular techniques, there has been a growing search for communities of commensal microorganisms, due to the fact that many of these populations have the capacity to prevent or favor many diseases. Thus, no consensus was found about the sterility of the mammary gland. some authors have assumed that the mammary gland has its own microbiota, while others argued that it is sterile. Regarding that, the objective of the present project is: 1) to define the best technique to collect material for the microbioma examination; 2) to evaluate the relationship between the integrity of the teat canal and the microbiota of the mammary gland of heifers, and its effect on the mammary gland health during the postpartum period; and 3) to investigate the existence of commensal microorganisms in nulliparous animals. For these reasons, this study will be divided into three phases. in phase 1, we will be use three forms of milk collection in multiparous animals, to define the best method of milk collection for the metagenomics analysis. Then, the microbiological and metagenomics analyses of the milk samples will be performed. For the second phase of this work, the opening of the teat canal during the prepartum period (21 days before parturition) in dairy heifers will be evaluated by visual inspection and ultrasonographic examination. Then, milk secretion will be collected for microbiological and metagenomics analyses. Furthermore, postpartum colostrum and milk samples will be collected at four moments for microbiological analysis and somatic cell count. Therefore, in the third phase, the existence of microbial populations in nulliparous animals will be investigated through mammary biopsy and metagenomics analysis. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARQUES, MICHELE FLAVIA SOUSA; DE MOURA, GUILHERME SANTANA; DE SOUZA, FERNANDO NOGUEIRA; GARGANO, RONALDO GOMES; SANTOS, KAMILA REIS; FERRONATTO, JOSE AUGUSTO; DELLA LIBERA, ALICE MARIA MELVILLE PAIVA; MOTA, RINALDO APARECIDO. Blood polymorphonuclear leukocyte responses against Staphylococcus aureus in primiparous and pluriparous Lacaune and Santa Ine circumflex accent s ewes. Small Ruminant Research, v. 201, AUG 2021. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.