Study of hybridization in Triatoma sordida (Stal, 1859) (Hemiptera, Reduviidae): evolutionary dynamics, reproductive viability, morpho-molecular characterization and its taxonomic implications

• Grant number: 17/05015-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): June 01, 2018
• Effective date (End): October 31, 2021
• Field of knowledge: Health Sciences - Collective Health - Epidemiology
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: João Aristeu da Rosa
• Grantee: Kaio Cesar Chaboli Alevi
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Triatoma sordida is one of the main species with vector competence for the transmission of Chagas' disease in Brazil, which also occurs in Argentina, Bolivia, Paraguay and Uruguay. Several studies indicate a polymorphism in T. sordida, which led to the suggestion of the cryptic speciation phenomenon in this species and, above all, the recent characterization of three possible different taxa, namely T. sordida sensu stricto, T. sordida Argentina and T. sordida La Paz. Taking into account that the accomplishment of experimental crosses can help in different aspects, such as the understanding of the taxonomy and systematics and the mechanisms of isolation that limit the gene flow, as well as to evaluate the role of natural hybridization in the generation of new genetic variants can lead to adaptive evolution and / or the foundation of new evolutionary lineages), this project aims at carrying out a multidisciplinary and integrated analysis of evolutionary (pre and post-zygotic barriers) and taxonomic in T. sordida, through experimental crosses and analysis of hybrids resulting to the F3, with emphasis on the analysis of the evolutionary dynamics of experimental crosses (copulation, oviposition, hatching and development of hybrids) as well as the analysis of reproductive viability (spermatogenesis and morphology of male and female gonads), the characterization of the nuclear and nucleolar organization of somatic cells, the pattern of segregation of phenotypic and genotypic characteristics of parental and vectorial capacity of experimental hybrids in the transmission of Trypanosoma cruzi. These results will allow elucidate evolutionary and taxonomic issues about T. sordida, and assist in the direction of vector control programs. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ANA BEATRIZ BORTOLOZO; TEDESCHI, BIANCA BARBERIO BOGDAN; DE OLIVEIRA, JADER; MADEIRA, FERNANDA FERNANDEZ; FALLEIROS JUNIOR, LUIZ ROBERTO; SILVA JUNIOR, FERNANDO CESAR; DA ROSA, JOAO ARISTEU; DE AZEREDO-OLIVEIRA, MARIA TERCILIA VILELA; ALEVI, KAIO CESAR CHABOLI. Parasite x vector relationship in Chagas disease: doesTrypanosoma cruzi(Chagas, 1909) infection affect the spermatogenesis ofTriatoma infestans(Klug, 1834)?. Parasitology Research, v. 119, n. 10, JUL 2020. (17/05015-7)

BELINTANI, TIAGO; OLIVEIRA, JADER; PINOTTI, HELOISA; SILVA, LUCAS ABRANTES; CHABOLI ALEVI, KAIO CESAR; GALVAO, CLEBER; DA ROSA, JOAO ARISTEU. Phylogenetic and phenotypic relationships of the Triatoma sordida subcomplex (Hemiptera: Reduviidae: Triatominae). Acta Tropica, v. 212, DEC 2020. (17/05015-7)

DOS REIS, YAGO VISINHO; CHABOLI ALEVI, KAIO CESAR. Hybridization in Phlebotominae (Diptera: Psychodidae): A mini-review. INFECTION GENETICS AND EVOLUTION, v. 86, DEC 2020. (17/05015-7)

BORTOLOZO DE OLIVEIRA, ANA BEATRIZ; RIBEIRO, ALINE RIMOLDI; MADEIRA, FERNANDA FERNANDEZ; CESARETTO, NATALIA REGINA; DA ROSA, JOAO ARISTEU; VILELA DE AZEREDO-OLIVEIRA, MARIA TERCILIA; CHABOLI ALEVI, KAIO CESAR. Cytotaxonomy of Trypanosoma cruzi (Chagas, 1909): Differentiation of T. cruzi I (TcI) and T. cruzi II (TcII) Genotypes Using Cytogenetic Markers. American Journal of Tropical Medicine and Hygiene, v. 101, n. 3, p. 605-607, 2019. (17/05015-7)

ALEVI, KAIO CESAR CHABOLI; DE OLIVEIRA, JADER; ROCHA, DAYSE DA SILVA; GALVAO, CLEBER. Trends in Taxonomy of Chagas Disease Vectors (Hemiptera, Reduviidae, Triatominae): From Linnaean to Integrative Taxonomy. PATHOGENS, v. 10, n. 12, DEC 2021. (17/05015-7, 19/02145-2)

GONZALEZ-BRITEZ, NILSA ELIZABETH; CHABOLI ALEVI, KAIO CESAR; CARIS GARCIA, ARIANE CRISTINA; MARTINEZ PURROY, CLARA ELENA; GALVAO, CLEBER; JOSE CARRASCO, HERNAN. Chagas Disease Vectors of Paraguay: Entomoepidemiological Aspects of Triatoma sordida (Stal, 1859) and Development of an Identification Key for Paraguayan Triatomines Based on Cytogenetics Data. American Journal of Tropical Medicine and Hygiene, v. 105, n. 1, p. 130-133, JUL 2021. (17/05015-7)

BELINTANI, TIAGO; OLIVEIRA, JADER; PINOTTI, HELOISA; CHABOLI ALEVI, KAIO CESAR; NASCIMENTO, JULIANA DAMIELI; SASSO-CERRI, ESTELA; GALVAO, CLEBER; DA ROSA, JOAO ARISTEU. Characterization of Female External Genitalia and Eggs of Four South American Species of the Triatoma Laporte, 1832 Genus (Hemiptera: Reduviidae: Triatominae). INSECTS, v. 12, n. 6, JUN 2021. (17/05015-7)

MADEIRA, FERNANDA FERNANDEZ; GRZYB DELGADO, LUIZA MARIA; BITTINELLI, ISADORA DE FREITAS; SARTORI, RICARDO QUITERIO; DE OLIVEIRA, JADER; DA ROSA, JOAO ARISTEU; VILELA DE AZEREDO-OLIVEIRA, MARIA TERCILIA; CHABOLI ALEVI, KAIO CESAR. Revisiting the genetic variability of Brazilian peridomestic populations of the Chagas disease vector Triatoma sordida (Stal 1859) (Hemiptera, Triatominae). INFECTION GENETICS AND EVOLUTION, v. 85, NOV 2020. (17/05015-7, 17/18046-8)

CHABOLI ALEVI, KAIO CESAR; DE OLIVEIRA, JADER; CANIS GARCIA, ARIANE CRISTINA; CRISTAL, DANIEL CESARETTO; GRZYB DELGADO, LUIZA MARIA; BITTINELLI, ISADORA DE FREITAS; DOS REIS, YAGO VISINHO; RAVAZI, AMANDA; BORTOLOZO DE OLIVEIRA, ANA BEATRIZ; GALVAO, CLEBER; et al. Triatoma rosai sp. nov. (Hemiptera, Triatominae): A New Species of Argentinian Chagas Disease Vector Described Based on Integrative Taxonomy. INSECTS, v. 11, n. 12, DEC 2020. (18/12039-2, 17/05015-7, 18/24116-1, 20/07917-0)

MADEIRA, FERNANDA FERNANDEZ; DOS REIS, YAGO VISINHO; BITTINELLI, ISADORA DE FREITAS; GRZYB DELGADO, LUIZA MARIA; DE OLIVEIRA, JADER; MENDONCA, VAGNER JOSE; FIGUEIREDO MOREIRA, FELIPE FERRAZ; VILELA DE AZEREDO-OLIVEIRA, MARIA TERCILIA; DA ROSA, JOAO ARISTEU; CHABOLI ALEVI, KAIO CESAR. Genetic Structure of Brazilian Populations of Triatoma sordida (Stal, 1859) (Hemiptera, Triatominae) by Means of Chromosomal Markers. American Journal of Tropical Medicine and Hygiene, v. 100, n. 4, p. 907-910, 2019. (17/05015-7, 18/12039-2, 14/07706-9, 13/19764-0)

VICENTE, ROBERTO DEZAN; MADEIRA, FERNANDA FERNANDEZ; BORSATTO, KELLY CRISTINE; CARIS GARCIA, ARIANE CRISTINA; CRISTAL, DANIEL CESARETTO; GRZYB DELGADO, LUIZA MARIA; BITTINELLI, ISADORA DE FREITAS; DE MELLO, DENIS VINICIUS; DOS REIS, YAGO VISINHO; RAVAZI, AMANDA; et al. Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae). PATHOGENS, v. 11, n. 11, p. 10-pg., 2022-11-01. (17/05015-7)

CARIS GARCIA, ARIANE CRISTINA; DE OLIVEIRA, JADER; CRISTAL, DANIEL CESARETTO; GRZYB DELGADO, LUIZA MARIA; BITTINELLI, ISADORA DE FREITAS; GALVAO, CLEBER; GONZALEZ BRITEZ, NILSA ELIZABETH; CARRASCO, HERNAN JOSE; DA ROSA, JOAO ARISTEU; CHABOLI ALEVI, KAIO CESAR. Intraspecific and Interspecific Phenotypic Differences Confirm the Absence of Cryptic Speciation in Triatoma sordida (Hemiptera, Triatominae). American Journal of Tropical Medicine and Hygiene, v. 105, n. 6, p. 1759-1766, DEC 2021. (19/17581-2, 18/24116-1, 17/05015-7, 18/12039-2)

PINOTTI, HELOISA; ALEVI, KAIO CESAR CHABOLI; DE OLIVEIRA, JADER; RAVAZI, AMANDA; MADEIRA, FERNANDA FERNANDEZ; REIS, YAGO VISINHO DOS; DE OLIVEIRA, ANA BEATRIZ BORTOLOZO; DE AZEREDO-OLIVEIRA, MARIA TERCILIA VILELA; DA ROSA, JOAO ARISTEU. Segregation of phenotypic characteristics in hybrids of Triatoma brasiliensis species complex (Hemiptera, Reduviidae, Triatominae). INFECTION GENETICS AND EVOLUTION, v. 91, JUL 2021. (17/05015-7)

MADEIRA, FERNANDA FERNANDEZ; GRZYB DELGADO, LUIZA MARIA; BITTINELLI, ISADORA DE FREITAS; DE OLIVEIRA, JADER; RAVAZI, AMANDA; DOS REIS, YAGO VISINHO; BORTOLOZO DE OLIVEIRA, ANA BEATRIZ; CRISTAL, DANIEL CESARETTO; GALVAO, CLEBER; VILELA DE AZEREDO-OLIVEIRA, MARIA TERCILIA; et al. Triatoma sordida (Hemiptera, Triatominae) from La Paz, Bolivia: an incipient species or an intraspecific chromosomal polymorphism?. PARASITES & VECTORS, v. 14, n. 1, OCT 27 2021. (17/05015-7, 19/17581-2, 18/12039-2, 18/24116-1)

PITA, S.; LORITE, P.; CUADRADO, A.; PANZERA, Y.; DE OLIVEIRA, J.; ALEVI, K. C. C.; ROSA, J. A.; FREITAS, S. P. C.; GOMEZ-PALACIO, A.; SOLARI, A.; et al. igh chromosomal mobility of rDNA clusters in holocentric chromosomes of Triatominae, vectors of Chagas disease (Hemiptera-Reduviidae. Medical and Veterinary Entomology, v. 36, n. 1, NOV 2021. (17/05015-7, 19/02145-2)