RGAs sugarcane expression profile during infection with various pathogens

• Grant number: 18/04555-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2018
• End date: April 10, 2022
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Claudia Barros Monteiro Vitorello
• Grantee: Hugo Rody Vianna Silva
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 16/17545-8 - Genes, genomes & transposable elements contribution to plant-microbe interaction: a sugarcane study case, AP.BIOEN.TEM
• Associated scholarship(s): 19/13530-4 - Modeling, analysis, and comparison of multiple sugarcane metabolic networks to unravel mechanisms of the smut disease progression, BE.EP.PD

Abstract

During the course of investigation of the pathosystem smut-sugarcane using a transcriptomic approach, resistance gene analogs were identified as differentially expressed (Schaker et al., 2016). A detailed analysis revealed that some of these genes are strong candidates to be associated with disease establishment in sugarcane. This previous work had provided a set of PacBio sequences of full length sugarcane transcripts and a second set of short length transcripts obtained by Illumina sequencing. This present project is to develop a database containing a combination of these sequencing data combining Illumina and PacBio sequenced to best define a set of sugarcane transcripts using the variety 'RB925345' as a model. The data will then be used to search potential copies and/or alleles of RGAs. The results will be validated by using qPCR techniques to identify gene copy number and also to provide an expression profile of each RGAs when the plant is challenged with different pathogens (Sporisorium scitaminuem, Leifsonia xyli, Xanthomonas albilineans) and endophytes (Gluconacetobacter). Questions that will be answered: 1) Is there a specific combination of RGAs expression for each pathogen detection?; 2) Is there a common path regarding RGA expression?; 3) Are there differences among RGAs expression in sugarcane varieties known as susceptible or resistant to smut or showing intermediate resistance? (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DAL'SASSO, THAIS CAROLINA DA SILVA; RODY, HUGO VIANNA SILVA; GRIJALBA, PABLO ENRIQUE; OLIVEIRA, LUIZ ORLANDO DE. Genome sequences and in silico effector mining of Corynespora cassiicola CC_29 and Corynespora olivacea CBS 114450. Archives of Microbiology, v. 203, n. 8, JUL 2021. (18/04555-0)

DAL'SASSO, THAIS C. S.; RODY, HUGO V. S.; OLIVEIRA, LUIZ O.. Genome-Wide Analysis and Evolutionary History of the Necrosis- and Ethylene-Inducing Peptide 1-Like Protein (NLP) Superfamily Across the Dothideomycetes Class of Fungi. Current Microbiology, v. 80, n. 1, p. 13-pg., 2023-01-01. (18/04555-0)

RODY, HUGO V. S.; BOMBARDELLI, RENATO G. H.; CRESTE, SILVANA; CAMARGO, LUIS E. A.; VAN SLUYS, MARIE-ANNE; MONTEIRO-VITORELLO, CLAUDIA B.. Genome survey of resistance gene analogs in sugarcane: genomic features and differential expression of the innate immune system from a smut-resistant genotype. BMC Genomics, v. 20, n. 1, NOV 6 2019. (18/04555-0, 16/17545-8)

TEIXEIRA-SILVA, NATALIA SOUSA; CARVALHO SCHAKER, PATRICIA DAYANE; SILVA RODY, HUGO VIANNA; MAIA, THIAGO; GARNER, CHRISTOPHER M.; GASSMANN, WALTER; MONTEIRO-VITORELLO, CLAUDIA BARROS. Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors. JOURNAL OF FUNGI, v. 6, n. 4, DEC 2020. (17/13268-2, 18/04555-0, 16/04429-0, 16/16376-8, 17/25477-5, 16/17545-8, 13/25599-2)

RODY, HUGO V. S.; CAMARGO, LUIS E. A.; CRESTE, SILVANA; VAN SLUYS, MARIE-ANNE; RIESEBERG, LOREN H.; MONTEIRO-VITORELLO, CLAUDIA B.. Arabidopsis-Based Dual-Layered Biological Network Analysis Elucidates Fully Modulated Pathways Related to Sugarcane Resistance on Biotrophic Pathogen Infection. FRONTIERS IN PLANT SCIENCE, v. 12, AUG 19 2021. (18/04555-0, 19/13530-4, 16/17545-8)