Combination of confocal fluorescence microscopy and surface-enhanced Raman scattering techniques: interaction, detection, and quantification of molecules of interest in biomimetic system.

• Grant number: 17/15019-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2018
• End date: October 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Carlos José Leopoldo Constantino
• Grantee: Cibely da Silva Martin Sonvesso
• Host Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 19/22872-6 - Pursuing nanomaterials inside of living cells, BE.EP.PD

Abstract

The images obtained by fluorescence microscopy are widely used in many researche areas, including with application well-established in clinical areas (diagnostic). On the other hand, the surface-enhanced Raman scattering (SERS) technique by micro-Raman equipment has attracted great interest to combine information at the molecular level (Raman spectrograph) and morphology (optical microscopy), allowing to present such results also in the form of images. Thus, the combination of fluorescence microscopy (confocal in this case) and micro-Raman/SERS can be an important tool for detection of molecules of biological, pharmacological and/or environmental interest. In addition, SERS analyzes can also be used for the identification (in relation to interferers) and quantification of molecules. Thus, this research project has as the main objective to develop a methodology that allows applying the combination of confocal fluorescence microscopy and micro-Raman/SERS techniques in a complementary way to the same object of analyses (same sample). The development of the proposed methodology will have as the main object the analysis of giant unilamellar vesicles (GUVs), which is widely applied as a mimetic system of the lipidic structure of cell membrane. To these GUVs will be incorporated gold nanoparticles (AuNps, coated or not) containing dopamine (DA), an important neurotransmitter of the central nervous system. The AuNps are necessary to enhance the Raman signal (SERS) and the DA will act as the target molecule to be detected/ quantified inside and outside the GUVs. The combination of these microscopic/spectroscopic techniques is scarcely reported in the literature, being the principal innovator/challenging element of this project, which can be established as an important tool for studies of surface/molecule interaction, as well as the detection and quantification of interest molecules in biomimetic systems, or even be extended for studies in vitro and in vivo systems

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARLIN, CIBELY S.; MAXIMINO, MATEUS D.; PEREIRA, MATHEUS S.; ALESSIO, PRISCILA. Polyphenol Detection in Mate Tea Samples Using Iron Tetrasulfonated Phthalocyanine Modified Screen-Printed Electrode. IEEE SENSORS JOURNAL, v. 19, n. 22, p. 10686-10692, NOV 15 2019. (17/15019-0, 17/06534-8)

MATHEUS S. PEREIRA; MATEUS D. MAXIMINO; CIBELY S. MARTIN; PEDRO H.B. AOKI; OSVALDO N. OLIVEIRA JR; PRISCILA ALESSIO. Lipid-matrix effects on tyrosinase immobilization in Langmuir and Langmuir-Blodgett films. Anais da Academia Brasileira de Ciências, v. 93, n. 1, 2021-03-26. (13/14262-7, 17/15019-0)

MARTIN, CIBELY S.; OLIVEIRA, MARCELO J. S.; MAXIMINO, MATEUS D.; PAZIN, WALLANCE M.; CONSTANTINO, CARLOS J. L.. ynergetic effect of silver nanoparticles and thiram on lipid bilayer. JOURNAL OF MOLECULAR LIQUIDS, v. 348, FEB 15 2022. (18/22214-6, 17/15019-0, 20/12129-1)

OLIVEIRA, TATIANA A.; MARTIN, CIBELY S.; RUBIRA, RAFAEL J. G.; DE BARROS, ANERISE; MAZALI, ITALO O.; CONSTANTINO, CARLOS J. L.. Influence of L-Dopa adsorption time and concentration on Au nanorods aggregation for SERS quantitative analysis. CURRENT APPLIED PHYSICS, v. 54, p. 7-pg., 2023-10-01. (22/04020-5, 17/15019-0, 20/05423-0, 18/22214-6)

MARTIN, CIBELY SILVA; KAVAZOI, HENRY SEITIRO; MIYAZAKI, CELINA MASSUMI; ALESSIO, PRISCILA; CONSTANTINO, CARLOS JOSE LEOPOLDO. Nickel Tetrasulfonated Phthalocyanine Decorated with AuNP as a Double Sensorial Platform: SERS and Electrochemical. CHEMOSENSORS, v. 11, n. 7, p. 15-pg., 2023-07-01. (20/11803-0, 18/22214-6, 17/15019-0, 14/50869-6)

OLIVEIRA, MARCELO J. S.; MARTIN, CIBELY S.; RUBIRA, RAFAEL J. G.; BATAGIN-NETO, AUGUSTO; CONSTANTINO, CARLOS J. L.; AROCA, RICARDO F.. Surface-enhanced Raman scattering of thiram: Quantitative and theoretical analyses. Journal of Raman Spectroscopy, v. 52, n. 12, SI, AUG 2021. (18/22214-6, 17/15019-0, 20/05423-0)

MARTIN, CIBELY S.; RUBIRA, RAFAEL J. G.; SILVA, JAQUELINE N.; ALESSIO, PRISCILA. Role of Iron Phthalocyanine Coordination in Catecholamines Detection. SURFACES, v. 4, n. 4, p. 323-335, DEC 2021. (17/15019-0, 14/50869-6, 17/06534-8, 20/05423-0)

FIGUEIREDO, MARIA L. B.; MARTIN, CIBELY S.; FURINI, LEONARDO N.; RUBIRA, RAFAEL J. G.; BATAGIN-NETO, AUGUSTO; ALESSIO, PRISCILA; CONSTANTINO, CARLOS J. L.. Surface-enhanced Raman scattering for dopamine in Ag colloid: Adsorption mechanism and detection in the presence of interfering species. Applied Surface Science, v. 522, AUG 30 2020. (14/50869-6, 16/09634-0, 17/15219-9, 17/15019-0)

KAVAZOI, HENRY S.; MARTIN, CIBELY S.; ALESSIO, PRISCILA. Comparative study of tetrasulfonated phthalocyanine modified screen-printed electrodes in paraquat. Synthetic Metals, v. 284, p. 10-pg., 2022-03-01. (18/22214-6, 17/15019-0, 14/50869-6, 17/06534-8)

MAXIMINO, MATEUS D.; SILVA, CARLA Y.; CAVALCANTE, DALITA G. S. M.; MARTIN, CIBELY S.; JOB, ALDO E.; OLIVEIRA, JR., OSVALDO N.; ALESSIO, PRISCILA. Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 203, JUL 2021. (18/22214-6, 17/06534-8, 17/15019-0)

KAVAZOI, HENRY S.; MARTIN, CIBELY S.; MAXIMINO, MATEUS D.; PEREIRA, MATHEUS S.; RODRIGUEZ-MENDEZ, MARIA L.; ALESSIO, PRISCILA. Controlling supramolecular structures in iron tetrasulfonated phthalocyanine films through pH variation: implications for thin-film device performance. Physical Chemistry Chemical Physics, v. N/A, p. 9-pg., 2023-09-20. (21/14235-6, 17/15019-0, 18/22214-6, 17/06534-8)

MAXIMINO, MATEUS D.; MARTIN, CIBELY S.; ALESSIO, PRISCILA. Bisphenol A exposure induces multiple effects in DOPC membrane models. JOURNAL OF MOLECULAR LIQUIDS, v. 359, p. 9-pg., 2022-08-01. (18/22214-6, 17/15019-0, 21/14235-6, 17/06534-8)

GARCIA, MELINA P. B.; MARTIN, CIBELY S.; KAVAZOI, HENRY S.; MAXIMINO, MATEUS D.; ALESSIO, PRISCILA. Perylene nanostructured films optimization for organic electronics: Molecular, structural, and electrochemical characterization. Thin Solid Films, v. 777, p. 10-pg., 2023-07-31. (21/14235-6, 17/15019-0, 18/22214-6, 14/50869-6)

MIYAZAKI, CELINA M.; MARTIN, CIBELY S.; CONSTANTINO, CARLOS J. L.. Gold conjugated-magnetite nanoparticles for magnetic concentration towards reproducibility and repeatability of SERS measurements. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 671, p. 9-pg., 2023-08-20. (20/11803-0, 18/22214-6, 17/15019-0, 14/50869-6)