Extracellular vesicles as potencial predictive marker in canine multicentric lymphoma

• Grant number: 17/15406-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2018
• End date: May 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Heidge Fukumasu
• Grantee: Taismara Kustro Garnica
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 14/02493-7 - Mammary tumors of dogs and the cancer stem cell theory: a comparative and translational approach, AP.JP
• Associated scholarship(s): 19/09510-8 - Investigating the role of exosomes derived from chemotherapy resistant lymphoma cells as mediators of cellular plasticity, BE.EP.MS

Abstract

The multicentric lymphoma is the most common hematopoieticneoplasia in dogs. The relapse is the major challenge and is related to patient's death.The extracellular vesicles (EV) and their miRNAs (Oncomirs), derived from tumor cellsare a source of information about the disease and is associate with relapse andmetastasis. The aim of this study is to evaluate the potential of the EV aspredictive markers in dogs with multicentric lymphoma submitted to CHOP protocol. Thirty plasma samples from healthy dogs andfifteen with multicentric lymphoma will use for isolation of EV and assess the differencesbetween size, concentration and Oncomirs profile. In lymphoma group samples will becollect prior to chemotherapy at each session of protocol and after finished protocol for180 days. The profile of Oncomirs will assessed in three times: before, in the middleand after one week at end. The isolation of EV will be by ultracentrifugation and themeasurement by the NanoSight microscope. The evaluation of Oncomirs will conductby RT-PCR. According to the literature, we expect to find an EV profile that iscorrelated to the lymphoma cells and that may be associated with disease progression,making it possible to use it as a marker.The development of predictive markers is a great opportunity to a better comprehension ofcellular communication involvement in cancer progression, in addition this tool can bethe solution for monitoring oncology patients in human and veterinary medicine. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARNICA, TAISMARA K.; LESBON, JESSIKA C. C.; AVILA, ANA C. F. C. M.; ROCHETTI, ARINA L.; MATIZ, OSCAR R. S.; RIBEIRO, ROANA C. S.; ZOPPA, ALINE; NISHIYA, ADRIANA T.; COSTA, MIRELA T.; DE NARDI, ANDRIGO B.; et al. Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas. SCIENTIFIC REPORTS, v. 10, n. 1, NOV 23 2020. (14/02493-7, 15/21829-9, 17/15406-3, 14/22887-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GARNICA, Taismara Kustro. The potential use of small extracellular vesicles as predictive marker in canine multicentric lymphoma. 2020. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.