Characterization of mechanism(s) molecular(s) involved in myotoxic effect of crota...
| Grant number: | 18/00014-5 |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| Effective date (Start): | May 01, 2018 |
| Effective date (End): | April 30, 2019 |
| Field of knowledge: | Biological Sciences - Pharmacology - General Pharmacology |
| Principal Investigator: | Mirian Akemi Furuie Hayashi |
| Grantee: | Giovanna Bello Papa |
| Home Institution: | Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). São Paulo , SP, Brazil |
Abstract Crotamine have been studied for some time and different biological activities were presented, although little is known about the molecular mechanism of action involved in its myotoxic effect, which was the first to be described and is characterized by the immobilization of the skeletal muscle of rodents. We have recently demonstrated that this effect involves the action of crotamine on sodium channels, preventing their closure, and direct action as a blocker of the potassium channels demonstrated by electrophysiology. In vivo pilot studies, performed by the group, point to the possibility that the action of crotamine is not only peripheral but also with possible central effects. Concomitant injection of crotamine and antipsychotic thioridazine in mice (in vivo) allowed to observe the potentiation of both immobilization effect on the hind limbs of rodents promoted by crotamine and sedative effect of thioridazine. Thus, we suggest here that these two compounds may present a common molecular pathway, and with relevance for the eventual identification of other molecular targets of crotamine in rodents. Therefore, it is expected in this study to better understand the mechanisms that lead to the hind limb immobilization effect, without involving the functioning of the front legs by exploring the influence determined by the presence of the antipsychotic on crotamine's effects. (AU) | |