Comparative Genetic Activity on B Chromosomes of Astyanax species (Teleostei, Characiformes)

• Grant number: 17/22447-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2018
• End date: October 31, 2021
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Fausto Foresti
• Grantee: Duílio Mazzoni Zerbinato de Andrade Silva
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

About 15% of eukaryotic organisms have extra chromosomes to the standard A complement called B chromosomes. These elements have long been considered as inert and poor elements in protein-coding genes. However, recent works have demonstrated the transcription of sequences from B chromosomes. Astyanax fish genus is an interesting model for the study of the origin and evolution of the B chromosomes. Studies of the Laboratório de Biologia e Genética de Peixes da UNESP of Botucatu (LBGP) demonstrate the occurrence of at least five protein-coding genes on the B chromosome of A. paranae, among which three presented differential expression between the gonads of females with 1B and 0B. In this sense, the present project intends to verify if the genes found in the B chromosome of A. paranae are also present in the B chromosomes of other species of Astyanax, as well as to verify the activity of these genes in these species; to investigate if the B chromosome of A. paranae can express its own genes and to analyze if the individuals with B chromosomes have differential expression of genes allocated in the A chromosomes. In this way, it will be possible to characterize the functional effects caused by the presence of the B chromosomes in A paranae; to verify if the B chromosomes present in different species of Astyanax have functional genes and to evaluate if the B chromosomes present in the species of the genus Astyanax had a common origin or if they are the result of independent origin processes.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZERBINATO DE ANDRADE SILVA, DUILIO MAZZONI; RUIZ-RUANO, FRANCISCO J.; UTSUNOMIA, RICARDO; MARTIN-PECINA, MARIA; PENA CASTRO, JONATHAN; PACCIELLI FREIRE, PAULA; FRANCISCO CARVALHO, ROBSON; HASHIMOTO, DIOGO T.; SUH, ALEXANDER; OLIVEIRA, CLAUDIO; et al. ong-term persistence of supernumerary B chromosomes in multiple species of Astyanax fis. BMC Biology, v. 19, n. 1, MAR 19 2021. (14/26508-3, 18/20610-1, 16/09204-6, 17/22447-8, 14/09634-5)

ZERBINATO DE ANDRADE SILVA, DUILIO MAZZONI; ARAYA-JAIME, CRISTIAN; YAMASHITA, MASAKANE; VIDAL, MATEUS ROSSETTO; OLIVEIRA, CLAUDIO; PORTO-FORESTI, FABIO; ARTONI, ROBERTO FERREIRA; FORESTI, FAUSTO. Meiotic self-pairing of the Psalidodon (Characiformes, Characidae) iso-B chromosome: A successful perpetuation mechanism. GENETICS AND MOLECULAR BIOLOGY, v. 44, n. 3, p. 8-pg., 2021-01-01. (18/20610-1, 16/09204-6, 14/09634-5, 17/22447-8, 17/15608-5, 14/26508-3)

DUÍLIO MAZZONI ZERBINATO DE ANDRADE SILVA; CRISTIAN ARAYA-JAIME; MASAKANE YAMASHITA; MATEUS ROSSETTO VIDAL; CLAUDIO OLIVEIRA; FÁBIO PORTO-FORESTI; ROBERTO FERREIRA ARTONI; FAUSTO FORESTI. Meiotic self-pairing of the Psalidodon (Characiformes, Characidae) iso-B chromosome: A successful perpetuation mechanism. GENETICS AND MOLECULAR BIOLOGY, v. 44, n. 3, 2021-10-04. (14/09634-5, 14/26508-3, 17/22447-8, 17/15608-5, 16/09204-6, 18/20610-1)

SILVA, DUILIO M. Z. A.; CASTRO, JONATHAN P.; GOES, CAIO A. G.; UTSUNOMIA, RICARDO; VIDAL, MATEUS R.; NASCIMENTO, CRISTIANO N.; LASMAR, LUCAS F.; PAIM, FABILENE G.; SOARES, LETICIA B.; OLIVEIRA, CLAUDIO; et al. B Chromosomes in Psalidodon scabripinnis (Characiformes, Characidae) Species Complex. ANIMALS, v. 12, n. 17, p. 19-pg., 2022-09-01. (16/09204-6, 19/15140-9, 17/22447-8, 14/26508-3, 20/01775-0, 18/20610-1)

BARBOSA, PATRICIA; SCHEMCZSSEN-GRAEFF, ZELINDA; MARQUES, ANDRE; DA SILVA, MAELIN; FAVERO, GIOVANI MARINO; SOBREIRO, BERNARDO PASSOS; DE ALMEIDA, MARA CRISTINA; MOREIRA-FILHO, ORLANDO; SILVA, DUILIO MAZZONI ZERBINATO DE ANDRADE; PORTO-FORESTI, FABIO; et al. Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis. CELLS, v. 10, n. 5, MAY 2021. (17/22447-8)

SILVA, MAELIN; SILVA, DUILIO MAZZONI ZERBINATO ANDRADE; CASTRO, JONATHAN PENA; MAKUNIN, ALEX I.; BARBY, FELIPE FAIX; DE OLIVEIRA, EDIVALDO HERCULANO CORREA; LIEHR, THOMAS; CIOFFI, MARCELO BELLO; PORTO-FORESTI, FABIO; FORESTI, FAUSTO; et al. Investigation of Astyanax mexicanus (Characiformes, Characidae) chromosome 1 structure reveals unmapped sequences and suggests conserved evolution. PLoS One, v. 19, n. 11, p. 14-pg., 2024-11-18. (17/22447-8)

ZERBINATO DE ANDRADE SILVA, DUILIO MAZZONI; RUIZ-RUANO, FRANCISCO J.; UTSUNOMIA, RICARDO; MARTIN-PECINA, MARIA; PENA CASTRO, JONATHAN; PACCIELLI FREIRE, PAULA; FRANCISCO CARVALHO, ROBSON; HASHIMOTO, DIOGO T.; SUH, ALEXANDER; OLIVEIRA, CLAUDIO; et al. Long-term persistence of supernumerary B chromosomes in multiple species of Astyanax fish. BMC Biology, v. 19, n. 1, p. 17-pg., 2021-03-19. (14/09634-5, 16/09204-6, 18/20610-1, 14/26508-3, 17/22447-8)

ANDRES ARAYA-JAIME, CRISTIAN; ZERBINATO DE ANDRADE SILVA, DUILIO MAZZONI; RIBEIRO DA SILVA, LUIS RICARDO; NEVES DO NASCIMENTO, CRISTIANO; OLIVEIRA, CLAUDIO; FORESTI, FAUSTO. Karyotype description and comparative chromosomal mapping of rDNA and U2 snDNA sequences in Eigenmannia limbata and E. microstoma (Teleostei, Gymnotiformes, Sternopygidae). COMPARATIVE CYTOGENETICS, v. 16, n. 2, p. 16-pg., 2022-05-05. (17/15608-5, 17/22447-8, 14/09634-5, 16/09204-6, 14/26508-3, 18/20610-1)

VIDAL, MATEUS ROSSETTO; LASMAR, LUCAS F.; NADAI, PAMELA C. F.; OLIVEIRA, CLAUDIO; SILVA, DUILIO M. Z. A.; FORESTI, FAUSTO. Selecting reference genes for RT-qPCR studies involving the presence of B chromosomes in Psalidodon (Characiformes, Characidae). MOLECULAR BIOLOGY REPORTS, v. 51, n. 1, p. 7-pg., 2024-12-01. (17/22447-8, 20/13433-6, 19/15140-9, 20/01775-0)