Fabrication of electrochemical sensors for ascorbate detection in the human pathogenic fungus Aspergillus fumigatus

• Grant number: 18/09027-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2018
• End date: July 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Mauro Bertotti
• Grantee: Vinícius Lima Furtado
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The focus of this research project is the determination of the concentration of ascorbate present in the cellular extract of the fungus Aspergillus fumigatus, using an electrochemical sensor developed specifically for this purpose. The sensor will consist of a gold microelectrode, which will have the surface treated in a special way aiming at the formation of nanostructures of the metal. At the modified surface the electron transfer process associated with the oxidation of ascorbate is expected to be facilitated, with a concurrent current enhancement. The project will be carried out in collaboration with the team of Prof. Luís Eduardo Soares Netto, from the Department of Genetics and Evolutionary Biology of the Institute of Biosciences, responsible for the supply of cellular extracts of the fungus A. Fumigatus.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KUMAR, ABHISHEK; FURTADO, VINICIUS L.; GONCALVES, JOSUE M.; BANNITZ-FERNANDES, RENATA; NETTO, LUIS EDUARDO S.; ARAKI, KOITI; BERTOTTI, MAURO. Amperometric microsensor based on nanoporous gold for ascorbic acid detection in highly acidic biological extracts. Analytica Chimica Acta, v. 1095, p. 61-70, JAN 25 2020. (18/08782-1, 18/21489-1, 18/09027-2)