Microtomography analysis of tissue repair of resinous endodontic sealers in critical surgical defects in rat calvaria

• Grant number: 18/03683-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2018
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Rodrigo Ricci Vivan
• Grantee: Gabriela Cristina de Santi Sodré
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The aim of this study will be to evaluate in vivo the tissue response and its capacity of bone repair of Sealer Plus and AH Plus® endodontic cements compared to the control group. The materials will be implanted in the calvaria of 48 Wistar rats, Split into groups of 8 animals for the experimental periods of 7 and 30 days. After each postoperative period, the animals will be euthanized and the calvarias will be removed and fixed in 10% formalin. Then, six pieces of each experimental period will be analyzed in Computerized Microtomography (MicroCT). The other pieces will be analyzed by eletronic scanning microscopy by energy dispersive spectroscopy (SEM/EDS). The microtomographic analyzes and SEM/EDS will reveal the bone neoformation and the elemental chemical composition of each material, respectively. The results for the newly formed volume will be analyzed by the ANOVA test and the elemental composition will be described in percentages for each sample, the level of significance between groups being 5% (p <0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUILHERME FERREIRA DA SILVA; LETYCIA ACCIOLY SIMÕES COELHO; VANESSA ABREU SANCHES COSTA; LETÍCIA CITELLI CONTI; ANA CAROLINA DE ALMEIDA LIMA; GABRIELA CRISTINA DE SANTI SODRÉ; MATEUS RINALDI LUCIO MARTINS; MARCO ANTONIO HUNGARO DUARTE; RODRIGO RICCI VIVAN. Laboratory study of tissue repair of resin-based endodontic sealers in critical surgical defects. Journal of Applied Oral Science, v. 30, 2022-08-05. (17/17248-6, 18/03683-5)