Rapid ligand screening models in natural products through ligand fishing assays and LC-HRMS

• Grant number: 18/11109-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2018
• End date: December 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Agreement: GlaxoSmithKline
• Principal Investigator: Quezia Bezerra Cass
• Grantee: Juliana Maria de Lima
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock, AP.PCPE
• Associated scholarship(s): 19/14629-4 - In-line liquid chromatography-native mass spectrometry method to screening bioactive compounds from natural sources, BE.EP.PD

Abstract

The identification of substances with biological activity in natural extracts or synthetic collections is a crucial step in the search for new drugs and requires the use of fast and efficient techniques of separation and screening. This fact encourages the development of new methods that facilitate and reduce the time spent on identifying such substances. The isolation of active principles derived from a natural extract can rarely be achieved in one step, thus strategies that integrate screening, separation and characterization are presented as promising tools in the search for new bioactive compounds. Thus, this project proposes the use of ligand fishing technique for the screening of plant extracts in search of bioactive compounds to human cholinesterases, coupled with high performance liquid chromatography and detection by high-resolution mass spectrometry. The species investigated in this work are plants of the Brazilian cerrado, selected based on ethnobotanical and chemotaxonomic knowledge. The project here presented is continuation of the work carried out by Dr. Mazzi-Leme.