Comparative diagnosis of canine visceral Leishmaniasis by the immunological and molecular technique

• Grant number: 18/13608-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2018
• End date: December 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Katia Denise Saraiva Bresciani
• Grantee: Francine Rodrigues Navarro
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Canine visceral leishmaniasis is a worldwide zoonosis. Brasil is considered the seventh country with the largest concentration of new cases of human disease. In endemic areas, and the domestic dog (Canis familiaris) the primary reservoir of this infection. The aim of this study will be to compare the diagnosis of visceral leishmaniasis by immunological and molecular techniques. Dogs (130) of the city of Araçatuba, SP, will be examined by serological analysis using enzyme-linked immunosorbent assay (ELISA) and Dual Plate Plataform (DPP - Bio-Manguinhos®) and molecular analysis using real-time PCR of the conjunctival swab. The statistical analysis will consist of descriptive and inferential statistics. The McNemar test will be used to compare the proportion of positive results from diagnostic techniques and Kappa coefficient of agreement. The statistics will be considered significant when p <0.05. The sensitivity and specificity values of the tests will be determined considering the sum of all diagnoses as the gold standard test.