Vulnerability to embolism in citrus species: hydraulic characteristics, seasonal variation and relationships between canopy and rootstock

• Grant number: 18/09834-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2019
• End date: June 30, 2023
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Rafael Vasconcelos Ribeiro
• Grantee: Marcela Trevenzoli Miranda
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated scholarship(s): 21/13329-7 - Vulnerability to embolism, gaseous dynamics and anatomical traits in Citrus plants, BE.EP.DR

Abstract

The practice of grafting affects the plant susceptibility to water deficit since rootstock and canopy exhibit different resistance to drought. In xylem vessels, the tension generated by transpiration can cause formation of embolism, which reduces the ability of plants to carry water. The vulnerability of branches to embolism varies throughout the growing season of plants as well as after a drought event. This study aims to evaluate the water relations of citrus species under field conditions and to reveal the structural and functional bases of embolims susceptibility, as well as to evaluate the association of these characteristics with the tolerance to drought in citrus. As hypotheses, we have that species and varieties of canopies grafted on the same rootstock present different hydraulic characteristics; rootstock species changes the susceptibility to embolism of canopies; hydraulic conductivity and vulnerability to embolism vary throughout the year, and the branches of trees with growing fruits have higher hydraulic conductivity. For this, we will do a study of hydraulic characterization of different canopy/rootstock combinations with importance for Brazil, besides a study of the seasonal variation of these characteristics in plants in the field. The hydraulic conductivity, hydraulic safety margin, anatomy, contribution of aquaporins to water transport, water potential and leaf gas exchange in orange trees of the Valencia, Pêra and Hamlin varieties, of Ponkan mandarin and of Tahiti lime based on Rangpur lime and Valencia sweet orange grafted on Rangpur lime, citrumelo Swingle and citrandarin. The selection of drought tolerant materials could be anticipated by understanding the characteristics that induce greater embolism resistance, this may be a tool used in citrus breeding programs. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, LUCIANO; BITTENCOURT, PAULO R. L.; ROWLAND, LUCY; BRUM, MAURO; MIRANDA, MARCELA T.; PACHECO, VINICIUS S.; OLIVEIRA, RAFAEL S.; MACHADO, EDUARDO C.; JANSEN, STEVEN; RIBEIRO, V, RAFAEL. Using the Pneumatic method to estimate embolism resistance in species with long vessels: A commentary on the article ``A comparison of five methods to assess embolism resistance in trees{''}. FOREST ECOLOGY AND MANAGEMENT, v. 479, JAN 1 2021. (17/14075-3, 18/09834-5)

PEREIRA, LUCIANO; MIRANDA, MARCELA T.; PIRES, GABRIEL S.; PACHECO, VINICIUS S.; GUAN, XINYI; KAACK, LUCIAN; KARIMI, ZOHREH; MACHADO, EDUARDO C.; JANSEN, STEVEN; TYREE, MELVIN T.; et al. A semi-automated method for measuring xylem vessel length distribution. THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY, v. 32, n. 4, p. 331-340, DEC 2020. (18/09834-5, 19/15276-8, 17/14075-3)

SILVA, SIMONE F.; MIRANDA, MARCELA T.; COSTA, VLADIMIR E.; MACHADO, EDUARDO C.; RIBEIRO, V, RAFAEL. Sink strength of citrus rootstocks under water deficit. TREE PHYSIOLOGY, v. 41, n. 8, p. 1372-1383, AUG 2021. (16/02199-7, 15/14817-4, 18/09834-5)

TRABI, CHRISTOPHE L.; PEREIRA, LUCIANO; GUAN, XINYI; MIRANDA, MARCELA T.; BITTENCOURT, PAULO R. L.; OLIVEIRA, RAFAEL S.; RIBEIRO, V, RAFAEL; JANSEN, STEVEN. A User Manual to Measure Gas Diffusion Kinetics in Plants: Pneumatron Construction, Operation, and Data Analysis. FRONTIERS IN PLANT SCIENCE, v. 12, JUN 7 2021. (17/14075-3, 18/09834-5, 19/15276-8)

ALMEIDA, R. L.; SILVEIRA, N. M.; MIRANDA, M. T.; PACHECO, V. S.; CRUZ, L. P.; XAVIER, M. A.; MACHADO, E. C.; RIBEIRO, R. V.. Evidence of photosynthetic acclimation to self-shading in sugarcane canopies. Photosynthetica, v. 60, n. 4, p. 8-pg., 2022-10-20. (18/09834-5, 19/27106-0, 19/06161-2, 19/08047-2)

JANSEN, S.; GUAN, X.; KAACK, L.; TRABI, C.; MIRANDA, M. T.; RIBEIRO, R., V; PEREIRA, L.; HOLTTA, T; SALMON, Y. The Pneumatron estimates xylem embolism resistance in angiosperms based on gas diffusion kinetics: a minireview. XI INTERNATIONAL WORKSHOP ON SAP FLOW, v. 1300, p. 8-pg., 2020-01-01. (17/14075-3, 18/09834-5)

PEREIRA, LUCIANO; BITTENCOURT, PAULO R. L.; PACHECO, VINICIUS S.; MIRANDA, MARCELA T.; ZHANG, YA; OLIVEIRA, RAFAEL S.; GROENENDIJK, PETER; MACHADO, EDUARDO C.; TYREE, MELVIN T.; JANSEN, STEVEN; et al. The Pneumatron: An automated pneumatic apparatus for estimating xylem vulnerability to embolism at high temporal resolutionKey-words. PLANT CELL AND ENVIRONMENT, v. 43, n. 1, p. 131-142, JAN 2020. (18/09834-5, 17/14075-3, 18/01847-0, 19/07773-1)

MIRANDA, MARCELA T.; ESPINOZA-NUNEZ, ERICK; SILVA, SIMONE F.; BOSCARIOL-CAMARGO, RAQUEL L.; MACHADO, EDUARDO C.; RIBEIRO, V, RAFAEL. eaf PIP2.1 and PIP2.5 are down-regulated in `Mandarin' lime under drough. THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY, v. 34, n. 1, JAN 2022. (18/09834-5, 11/16263-5, 19/15276-8)

MIRANDA, MARCELA T.; ESPINOZA-NUNEZ, ERICK; SILVA, SIMONE F.; PEREIRA, LUCIANO; HAYASHI, ADRIANA H.; BOSCARIOL-CAMARGO, RAQUEL L.; CARVALHO, SERGIO A.; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL, V. Water stress signaling and hydraulic traits in three congeneric citrus species under water deficit. Plant Science, v. 319, p. 9-pg., 2022-03-22. (18/09834-5, 19/15276-8, 11/16263-5)

PEREIRA, LUCIANO; BITTENCOURT, PAULO R. L.; ROWLAND, LUCY; BRUM, MAURO; MIRANDA, MARCELA T.; PACHECO, VINICIUS S.; OLIVEIRA, RAFAEL S.; MACHADO, EDUARDO C.; JANSEN, STEVEN; RIBEIRO, RAFAEL, V. Using the Pneumatic method to estimate embolism resistance in species with long vessels: A commentary on the article "A comparison of five methods to assess embolism resistance in trees". FOREST ECOLOGY AND MANAGEMENT, v. 479, p. 3-pg., 2021-01-01. (18/09834-5, 17/14075-3)