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Development and characterization of scaffolds based on PCL - PGE and DNA applied to tissue engineering for bone regeneration

Grant number: 18/10237-1
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2019
Effective date (End): December 31, 2020
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Antonio Claudio Tedesco
Grantee:Cristiano Ceron Jayme
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The present project will use the combination of different materials to advocate the construction of scaffold using polycaprolactone (PCL) and polyethylene glycol (PEG) combined with a deoxyribonucleic acid (DNA) hydrogel applied as a human fetal osteoblast cellular transport system (hFOB 1.19) ATCC (CRL-11372), used in bone regeneration. In addition, a second strategy of direct application under the scaffolds of mesenchymal stem cell lines derived from human bone marrow (HBMS) ATCC (PCS-500-012) will be used to obtain functional osteoblasts. aroused great interest due to their potential involvement in the tissue repair process. DNA-based hydrogels have the function of improving the cellular interaction with pre-fabricated scaffolds, homogeneously distributing the cells and thus mimicking the complex structure of the tissues in addition to improving the conditions of cellular interaction, limited in PCL-PEG scaffolds . In a previous work, it was demonstrated the potential of DNA application as high biocompatibility material, allowing the application to regenerative medicine. Thus, in the present project, we aim to: (i) optimize, evaluate the interaction conditions of hydrogel-cells and the molecular mechanisms of interaction and adhesion between hFOB cell line 1.19 and scaffold; (ii) to differentiate the lineage of mesenchymal stem cells (HBMS) leading to the formation of osteoblasts under the scaffold and preceding the development of scaffolds with effective clinical and experimental application and the possibility of commercial use of the present product.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
REZENDE, NAYARA; JAYME, CRISTIANO CERON; BRASSESCO, MARIA SOL; TEDESCO, ANTONIO CLAUDIO; DE OLIVEIRA, HARLEY FRANCISCO. Standardization of a resazurin-based assay for the evaluation of metabolic activity in oral squamous carcinoma and glioblastoma cells. Photodiagnosis and Photodynamic Therapy, v. 26, p. 371-374, JUN 2019. Web of Science Citations: 2.
CALORI, ITALO RODRIGO; JAYME, CRISTIANO CERON; UENO, LEONARDO TSUYOSHI; CORRETO MACHADO, FRANCISCO BOLIVAR; TEDESCO, ANTONIO CLAUDIO. Theoretical and experimental studies concerning monomer/aggregates equilibrium of zinc phthalocyanine for future photodynamic action. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 214, p. 513-521, MAY 5 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.