Cholesterol-rich nanoemulsion (LDE) as a novel drug delivery system to diagnose, delineate, and treat human glioblastoma

Author(s)

Tedesco, Antonio Claudio ^[1] ; Silva, Emanoel P. O. ^[1] ; Jayme, Cristiano C. ^[1] ; Piva, Henrique L. ^[1] ; Franchi, Leonardo P. ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Photobiol & Photomed Res Grp, Dept Chem, Ctr Nanotechnol & Tissue Engn, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Goias UFG, Dept Bioquim & Biol Mol, Inst Ciencias Biol ICB 2, Campus Samambaia, BR-74690900 Goiania, Go - Brazil

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 123, APR 2021.
• Web of Science Citations: 0

Abstract

We have prepared and characterized a cholesterol-rich nanoemulsion called LDE, a mimic of classic lipoprotein macromolecules, that can be applied as a new drug delivery system for aluminum phthalocyanine chloride (PcAlCl). The LDE containing PcAlCl system prepared herein had mean size and zeta potential of 127 nm and -29 mV, respectively, and encapsulation rate efficiency was 81%, and stability of 17 months. Compared to classical liposomes, LDE was more efficient, especially in brain diseases like glioblastoma (GBM), as revealed by tests on the U-87 MG cell line. The LDEPc formulation did not display dark cytotoxicity, as expected. The best light dose for LDEPc was 1.0 J?cm? 2: its activity was 55% higher than PcAlCl in a compatible organic medium. In the U-87 MG cells, apoptosis was the preferential pathway activated by PDT. These results strongly support the use of LDE as a new theranostic system. (AU)

• FAPESP's process: 12/25216-3 - Photophysical and photobiological studies of drug delivery systems for photosensitizer application in photodynamic therapy
• Grantee: Emanoel Pedro de Oliveira Silva
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/10237-1 - Development and characterization of scaffolds based on PCL-PGE and DNA applied to tissue engineering for bone regeneration
• Grantee: Cristiano Ceron Jayme
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 13/50181-1 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders
• Grantee: Antonio Claudio Tedesco
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 14/11870-9 - Sensitizing cancer cell lines to photodynamic therapy through inhibition of protein APE1
• Grantee: Leonardo Pereira Franchi
• Support type: Scholarships in Brazil - Post-Doctorate