Photophysical and photobiological studies of drug delivery systems for photosensitizer application in photodynamic therapy

• Grant number: 12/25216-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2013
• End date: February 29, 2016
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Antonio Claudio Tedesco
• Grantee: Emanoel Pedro de Oliveira Silva
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Malignant gliomas are the most common brain tumor type, occurring in 5-7 per 100,000 individuals per year. The most frequent form of glioma is known as glioblastom multiform a (GBM), which occurs in 60-70% of cases. This tumor type is characterized by a fast aggressive growing and by the ability to infiltrate the surrounding healthy brain tissue, making it difficult to visualize the tumor contours and therefore it is necessary to the total removal of the tumor region is always associated with a poor prognosis. The current treatment is surgical resection followed by radiation treatment and chemotherapy. However, the glioma cells have been exhibited resistance to radiation and chemotherapy. Photodynamic therapy (PDT) has been an alternative for the treatment of brain tumors, and has shown promising results, both in vitro and in vivo, is a simple and non-evasive technique. This project proposes the preparation of a liposome maid of L-alpha phosphatidylcholine (Lectin) and nanoemulsions LDE in the presence of a phthalocyanine derivative as delivery system in photodynamic therapy. The photophysics and photochemistry characteristics of the delivery systems will be performed by using steady-state techniques (absorption and fluorescence) and time resolved (singlet and triplet lifetime determination and laser flash photolysis) evaluating the delivery systems influence on photophysical characteristics of the phthalocyanine derivative. In addition, photobiological studies will be conducted with both the delivery systems of phthalocyanine derivative and phthalocyanine derivative alone, assessing the possible differences between them using the glioma cell line (U87) as a biological model. As results, by using these delivery systems is expected a higher uptake of photosensitive agent by the glioma cells, thus enhancing its photodynamic efficiency. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TEDESCO, ANTONIO CLAUDIO; SILVA, EMANOEL P. O.; JAYME, CRISTIANO C.; PIVA, HENRIQUE L.; FRANCHI, LEONARDO P.. Cholesterol-rich nanoemulsion (LDE) as a novel drug delivery system to diagnose, delineate, and treat human glioblastoma. Materials Science & Engineering C-Materials for Biological Applications, v. 123, APR 2021. (12/25216-3, 18/10237-1, 13/50181-1, 14/11870-9)

SILVA, E. P. O.; FRANCHI, L. P.; TEDESCO, A. C.. Chloro-aluminium phthalocyanine loaded in ultradeformable liposomes for photobiology studies on human glioblastoma. RSC ADVANCES, v. 6, n. 83, p. 79631-79640, 2016. (12/25216-3, 13/50181-1, 14/11870-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Emanoel Pedro de Oliveira. Photophysical and photobiological studies of drug delivery systems loaded with chloro-aluminium phthalocyanine for application in photodynamic therapy. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.