New approaches in metabolomics: 3D Molecular cartography of ascidians based on LC-MS/MS data

• Grant number: 18/24865-4
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: March 01, 2019
• End date: February 29, 2020
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Leticia Veras Costa Lotufo
• Grantee: Anelize Bauermeister
• Supervisor: Pieter C Dorrestein
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of California, San Diego (UC San Diego), United States
• Associated to the scholarship: 17/17648-4 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds, BP.PD

Abstract

Ascidians (Tunicata), or sea squirts, are benthic marine invertebrates widely investigated as a source of new chemical structures with pharmacological potential. More than 1000 molecules were described from ascidians so far. However, the production of most of these metabolites was latter attributed to associated-bacteria, which live a syntrophic association with the tunicate. This relationship between associated-bacteria and their host still is a universe very poorly understood, and efforts on the investigation of this interaction should be encouraged. There are many published works in high impact journal investigating the microbiota associated to ascidians, however, most of them collect the animal and process it entirely, using this mixture as a source of microbes and metabolites. Nonetheless, ascidians are organisms with organized systems, including ganglion, stomach, intestine, heart, a tunic that covers the body, among others. Considering that, some questions arises: (i) how does the interaction of ascidians and their microbiota influences the secondary metabolism? (ii) how is the microbiota distributed through the ascidians body? To get some insights regarding these issues, we would like to investigate the metabolic and microbial profile of different organs and tissues from ascidians body by employing mass spectrometry based metabolomic approaches. We believe that investigations that cover different aspects of this research field remains of extremely importance for science. Mass spectrometry (MS) is a versatile and very sensitive technique that when coupled to chromatography allows the analyses and detection of metabolites in complex samples, even metabolites at low concentration, very important characteristics to investigate biological samples. The MS data will be analyzed by Molecular network, an online molecular tool, able to grouping the compounds from the entire data set by structural similarity, besides helping in the identification of known compounds in the sample. Moreover, here, we are proposing the use of such MS data in order to construct a 3D volume model to better visualize the distribution of metabolites in ascidians body. Such approach, recently developed by Pieter Dorrestein's group (data not published, in revision at Nature), allows the visualization of molecular distribution through an organism's body, considering separately each part, laying a foundation for studies of metabolism and microbial-host-environment interaction.

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAHM, BIANCA DEL B.; PERES, JADE; REZENDE-TEIXEIRA, PAULA; SANTOS, EVELYNE A.; BRANCO, PAOLA C.; BAUERMEISTER, ANELIZE; KIMANI, SERAH; MOREIRA, EDUARDA A.; BISI-ALVES, RENATA; BELLIS, CLAIRE; et al. Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins. FRONTIERS IN CHEMISTRY, v. 8, MAR 3 2020. (18/24865-4, 15/17177-6, 19/02008-5, 18/08400-1, 17/09022-8)

MANNOCHIO-RUSSO, HELENA; BUENO, PAULA CAROLINA P.; BAUERMEISTER, ANELIZE; DE ALMEIDA, RAFAEL FELIPE; DORRESTEIN, PIETER C.; CAVALHEIRO, ALBERTO JOSE; BOLZANI, VANDERLAN S.. Can Statistical Evaluation Tools for Chromatographic Method Development Assist in the Natural Products Workflow? A Case Study on Selected Species of the Plant Family Malpighiaceae. Journal of Natural Products, v. 83, n. 11, p. 3239-3249, NOV 25 2020. (19/08477-7, 17/19702-6, 18/24865-4, 13/07600-3, 16/16970-7, 14/50926-0)

FURTADO, LUCIANA C.; BAUERMEISTER, ANELIZE; DE FELICIO, RAFAEL; ORTEGA, RAQUEL; PINTO, FRANCISCO DAS CHAGAS L.; MACHADO-NETO, JOAO AGOSTINHO; TRIVELLA, DANIELA B. B.; PESSOA, OTILIA D. L.; WILKE, DIEGO V.; LOPES, NORBERTO P.; et al. Marine Streptomyces sp. Isolated From the Brazilian Endemic Tunicate Euherdmania sp. Produces Dihydroeponemycin and Analogs With Potent Antiglioma Activity. FRONTIERS IN MARINE SCIENCE, v. 8, MAY 3 2021. (18/24865-4, 17/18235-5, 15/17177-6, 17/17648-4)

SCHMID, ROBIN; PETRAS, DANIEL; NOTHIAS, LOUIS-FELIX; WANG, MINGXUN; ARON, ALLEGRA T.; JAGELS, ANNIKA; TSUGAWA, HIROSHI; RAINER, JOHANNES; GARCIA-ALOY, MAR; DUHRKOP, KAI; et al. Ion identity molecular networking for mass spectrometry-based metabolomics in the GNPS environment. NATURE COMMUNICATIONS, v. 12, n. 1, JUN 22 2021. (18/24865-4, 19/06061-8)

JARMUSCH, ALAN K.; WANG, MINGXUN; ACEVES, CHRISTINE M.; ADVANI, ROHIT S.; AGUIRRE, SHADEN; AKSENOV, ALEXANDER A.; ALETI, GAJENDER; ARON, ALLEGRA T.; BAUERMEISTER, ANELIZE; BOLLEDDU, SANJANA; et al. ReDU: a framework to find and reanalyze public mass spectrometry data. NATURE METHODS, v. 17, n. 9, AUG 2020. (18/24865-4)

MANNOCHIO-RUSSO, HELENA; DE ALMEIDA, RAFAEL F.; NUNES, WILHAN D. G.; BUENO, PAULA C. P.; CARABALLO-RODRIGUEZ, ANDRES M.; BAUERMEISTER, ANELIZE; DORRESTEIN, PIETER C.; BOLZANI, VANDERLAN S.. Untargeted Metabolomics Sheds Light on the Diversity of Major Classes of Secondary Metabolites in the Malpighiaceae Botanical Family. FRONTIERS IN PLANT SCIENCE, v. 13, p. 19-pg., 2022-04-14. (13/07600-3, 18/24865-4, 14/50926-0, 17/19702-6, 19/08477-7)

RICARDO MOREIRA BORGES; JOÃO VICTOR MENDES RESENDE; ALDEBARAN OLIVEIRA DE MORAES; ALANA KELYENE PEREIRA; RAFAEL GARRETT; ANELIZE BAUERMEISTER; ANTONIO JORGE RIBEIRO DA SILVA. GUIA PARA PROCESSAMENTO DE DADOS DE CROMATOGRAFIA ACOPLADA A ESPECTROMETRIA DE MASSAS. Química Nova, v. 45, n. 5, p. 608-620, 2022-07-22. (18/24865-4)

BAUERMEISTER, ANELIZE; MANNOCHIO-RUSSO, HELENA; COSTA-LOTUFO, LETICIA V.; JARMUSCH, ALAN K.; DORRESTEIN, PIETER C.. ass spectrometry-based metabolomics in microbiome investigation. NATURE REVIEWS MICROBIOLOGY, v. 20, n. 3, SEP 2021. (15/17177-6, 18/24865-4, 18/07098-0)

MOREIRA, EDUARDA ANTUNES; REZENDE-TEIXEIRA, PAULA; ALBERNAZ, LORENA CARNEIRO; BAUERMEISTER, ANELIZE; JIMENEZ, PAULA CHRISTINE; ESPINDOLA, LAILA SALMEN; COSTA-LOTUFO, LETICIA VERAS; LOPES, NORBERTO PEPORINE. Marine Bacteria from the Southeast Coast of Brazil as a Source of Insecticidal Compounds. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 32, n. 5, p. 10-pg., 2022-08-26. (15/17177-6, 18/24865-4, 17/17648-4, 14/50926-0)

SAHM, BIANCA DEL B.; PERES, JADE; REZENDE-TEIXEIRA, PAULA; SANTOS, EVELYNE A.; BRANCO, PAOLA C.; BAUERMEISTER, ANELIZE; KIMANI, SERAH; MOREIRA, EDUARDA A.; BISI-ALVES, RENATA; BELLIS, CLAIRE; et al. Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins. RONTIERS IN CHEMISTR, v. 8, p. 9-pg., 2020-03-03. (17/09022-8, 18/24865-4, 18/08400-1, 15/17177-6, 19/02008-5)