Method development and prospection studies of marine biological resources with citotoxic potential based on systems biology

• Grant number: 14/12343-2
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): September 01, 2014
• Effective date (End): September 02, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal researcher: Norberto Peporine Lopes
• Grantee: Fausto Carnevale Neto
• Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 16/22573-0 - Functional dereplication of marine natural products by integrative high-content image-based cytological screening and computational-assisted untargeted metabolomics, BE.EP.PD

Abstract

The search for bioactive substances in natural products has stimulated integrated approaches capable of providing information about the processes and interactions involved in the pharmacological responses of bioactive matrices. Among these approaches, highlights the metabolomics which, from the qualitative and quantitative analysis of the molecular composition of an organism, allows the explanation of biological processes through evaluation of metabolic responses, as well as an integrated view of the biochemistry of a complex mixture and its biological effects on a specific organism. This project aims to develop methods to assess the cytotoxic potential and the possible mechanisms involved in the bioactivity by integrated analysis of the metabolite profile of different tumor cell lines against complex matrices from brazilian marine organisms. The metabolic analyses will involve the use of mass spectrometry (MS) coupled with different chromatographic methods, chemometric tools for data interpretation (PCA, PLS-DA).

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VAN SANTEN, JEFFREY A.; JACOB, GREGOIRE; SINGH, AMRIT LEEN; ANIEBOK, VICTOR; BALUNAS, MARCY J.; BUNSKO, DEREK; CARNEVALE NETO, FAUSTO; CASTANO-ESPRIU, LAIA; CHANG, CHEN; CLARK, TREVOR N.; LITTLE, JESSICA L. CLEARY; DELGADILLO, DAVID A.; DORRESTEIN, PIETER C.; DUNCAN, KATHERINE R.; EGAN, JOSEPH M.; GALEY, MELISSA M.; HAECKL, F. P. JAKE; HUA, ALEX; HUGHES, ALISON H.; ISKAKOVA, DASHA; KHADILKAR, ASWAD; LEE, JUNG-HO; LEE, SANGHOON; LEGROW, NICOLE; LIU, DENNIS Y.; MACHO, JOCELYN M.; MCCAUGHEY, CATHERINE S.; MEDEMA, MARNIX H.; NEUPANE, RAM P.; O'DONNELL, TIMOTHY J.; PAULA, JASMINE S.; SANCHEZ, LAURA M.; SHAIKH, ANAM F.; SOLDATOU, SYLVIA; TERLOUW, BARBARA R.; TRAN, TUAN ANH; VALENTINE, MERCIA; VAN DER HOOFT, JUSTIN J. J.; VO, DUY A.; WANG, MINGXUN; WILSON, DARRYL; ZINK, KATHERINE E.; LININGTON, ROGER G. The Natural Products Atlas: An Open Access Knowledge Base for Microbial Natural Products Discovery. ACS CENTRAL SCIENCE, v. 5, n. 11, p. 1824-1833, NOV 27 2019. Web of Science Citations: 0.

NETO, FAUSTO CARNEVALE; ANDREO, MARCIO ADRIANO; RAFTERY, DANIEL; CALLEGARI LOPES, JOAO LUIS; LOPES, NORBERTO PEPORINE; CASTRO-GAMBOA, IAN; DE NORONHA SALES MAIA, BEATRIZ HELENA LAMEIRO; COSTA, EMMANOEL VILACA; VESSECCHI, RICARDO. Characterization of aporphine alkaloids by electrospray ionization tandem mass spectrometry and density functional theory calculations. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v. 34, n. 3, SI OCT 2019. Web of Science Citations: 5.

PILON, ALAN CESAR; GU, HAIWEI; RAFTERY, DANIEL; BOLZANI, VANDERLAN DA SILVA; LOPES, NORBERTO PEPORINE; CASTRO-GAMBOA, IAN; CARNEVALE NETO, FAUSTO. Mass Spectral Similarity Networking and Gas-Phase Fragmentation Reactions in the Structural Analysis of Flavonoid Glycoconjugates. Analytical Chemistry, v. 91, n. 16, p. 10413-10423, AUG 20 2019. Web of Science Citations: 2.

BRUNETTI, A. E.; CARNEVALE NETO, F.; VERA, M. C.; TABOADA, C.; PAVARINI, D. P.; BAUERMEISTER, A.; LOPES, N. P. An integrative omics perspective for the analysis of chemical signals in ecological interactions. CHEMICAL SOCIETY REVIEWS, v. 47, n. 5, p. 1574-1591, MAR 7 2018. Web of Science Citations: 24.

CESAR, FRANCINE C. S.; CARNEVALE NETO, FAUSTO; PORTO, GECIANE S.; MAIA CAMPOS, PATRICIA M. B. G. PATENT ANALYSIS: A LOOK AT THE INNOVATIVE NATURE OF PLANT-BASED COSMETICS. Química Nova, v. 40, n. 7, p. 840-847, AUG 2017. Web of Science Citations: 3.

DE OLIVEIRA, GIBSON GOMES; CARNEVALE NETO, FAUSTO; DEMARQUE, DANIEL PECORARO; DE SOUSA PEREIRA-JUNIOR, JOSE ANTONIO; SAMPAIO PEIXOTO FILHO, ROMULO CESAR; DE MELO, SEBASTIAO JOSE; GUEDES DA SILVA ALMEIDA, JACKSON ROBERTO; CALLEGARI LOPES, JOAO LUIZ; LOPES, NORBERTO PEPORINE. Dereplication of Flavonoid Glycoconjugates from Adenocalymma imperatoris-maximilianii by Untargeted Tandem Mass Spectrometry-Based Molecular Networking. Planta Medica, v. 83, n. 7, p. 636-646, MAY 2017. Web of Science Citations: 4.

TABOADA, CARLOS; BRUNETTI, ANDRES E.; PEDRON, FEDERICO N.; NETO, FAUSTO CARNEVALE; ESTRIN, DARIO A.; BARI, SARA E.; CHEMES, LUCA B.; LOPES, NORBERTO PEPORINE; LAGORIO, MARIA G.; FAIVOVICH, JULIAN. Naturally occurring fluorescence in frogs. Proceedings of the National Academy of Sciences of the United States of America, v. 114, n. 14, p. 3672-3677, APR 4 2017. Web of Science Citations: 25.

CARNEVALE NETO, FAUSTO; GUARATINI, THAIS; COSTA-LOTUFO, LETICIA; COLEPICOLO, PIO; GATES, PAUL J.; LOPES, NORBERTO PEPORINE. Re-investigation of the fragmentation of protonated carotenoids by electrospray ionization and nanospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry, v. 30, n. 13, p. 1540-1548, JUL 15 2016. Web of Science Citations: 5.