Antioxidant enzymatic, non-enzymatic defenses and lipid oxidative damage in the submandibular glands of rats treated with an anti-obesity drug

• Grant number: 18/21479-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2019
• End date: November 30, 2019
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Antonio Hernandes Chaves Neto
• Grantee: Henrique Arnaldo de Oliveira
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Sibutramine hydrochloride (SIBU) is a selective inhibitor of norepinephrine and serotonin reuptake with anorectic and thermogenic action. The most frequent side effects are dysgeusia and xerostomia. Our studies have shown that SIBU increases the relative and absolute weight and mucin levels of the submandibular glands (SM). The antioxidant defense imbalance may be associated with salivary gland dysfunctions. The objective of this work is to evaluate the non-enzymatic antioxidant defense by total iron reduction antioxidant power (FRAP), reduced glutathione (GSH) and uric acid (AU), besides the enzymatic defense that will be evaluated by superoxide dismutase (SOD) , catalase (CAT) and glutathione peroxidase (GPx) in the SM glands of SIBU treated rats. The analysis of thiobarbituric acid reactive substances (TBARs) will be used as a marker of lipid oxidative damages. The work was authorized by CEUA Local (FOA Protocol / UNESP n ° 00301-2016). Twenty-four Wistar rats (4 months / 350-450 g) will be divided into three groups and treated for 28 consecutive days by intragastric gavage with 6 and 10 mg / kg body weight of SIBU, SBU6 and SBU10, respectively, and the group Control (CON), which will only receive dimethylsulfoxide vehicle and saline solution during the same period. At the end of the treatment, the animals will be weighed and euthanized by exsanguination under general anesthesia. The SM glands will be removed and sequentially stored at -80øC until analysis. The normality and homoscedasticity of the data will be analyzed and they will be submitted to the most suitable parametric or non-parametric test. For all tests, the rejection level of the null hypothesis will be set at 5% (p <0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, DAMARIS RAISSA; FIAIS, GABRIELA ALICE; OLIVEIRA, HENRIQUE ARNALDO; RIBAS, TAYNA BUFFULIN; SOUZA, RAYNE OLIVEIRA; SAORI TSOSURA, THAIS VERONICA; MATSUSHITA, DORIS HISSAKO; ERVOLINO, EDILSON; MENEGATI DORNELLES, RITA CASSIA; DE MELO STEVANATO NAKAMUNE, ANA CLAUDIA; et al. Assessment of redox state and biochemical parameters of salivary glands in rats treated with anti-obesity drug sibutramine hydrochloride. CLINICAL ORAL INVESTIGATIONS, v. N/A, p. 14-pg., 2022-05-13. (18/21479-6)