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Evaluation of pulp capping materials on viability of different cell lines of dental pulp of deciduous teeth

Grant number: 18/19048-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2019
Effective date (End): January 31, 2020
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Natalino Lourenço Neto
Grantee:Karina Luisa Azanha Aquino
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The objective of the present project will be to compare the cell viability of different pulp capping materials against two cell lines derived from the pulp of deciduous human teeth: stem cells from deciduous exfoliated teeth (SHED) and pulp fibroblasts (HPF). The two SHED and HPF cell types will be obtained from existing biorrepositories at the Bauru Dental School (FOB-USP), and these cells have already been properly characterized. The cells will be thawed and expanded until the required amount of cells is obtained for the proposed experiments. The cell viability test will be based on ISO 10993-5 by treating the cells with conditione cell culture medium obtained from the different capping materials according to the following experimental groups: Group 1: MTA; Group 2: Portland cement; Group 3: Membrane; Control: Without the addition of the conditioned medium. After the experimental periods of 6 12 and 24 hours of contact with the extracts, the MTT Assay will be carried out to evaluate cell viability. The results obtained will be analyzed by the ANOVA method at two criteria followed by the Tukey test. A significance level of 5% will be adopted for differences to be considered statistically significant.