Effect of the heterologous, homologous and autologous seminal plasma on the sperm viability of stallions SEMEM submitted to refrigeration

• Grant number: 18/02451-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2018
• End date: November 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Frederico Ozanam Papa
• Grantee: Carolina Vicente de Aquino Pereira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The use of reproductive biotechniques, especially cryopreservation of equine semen, is constantly growing. However, it needs constant improvement to increase fertility rates in some stallions that are still very low. These stallions are called as refrigeration sensitive, so there is a constant interest in the search for improvements in techniques, processes, and manipulation to raise the fertility rates of the cryopreserved semen of these animals. An assumption quite mentioned in the literature is the seminal plasma quality of this group of animals. In this sense, studies have been used over the years to replace the seminal plasma with the seminal plasma of stallions called cryopreservation resistance. Despite the results found, there is still a great variation regarding the replacement of seminal plasma between different stallions. On the other hand, the seminal plasma of the donkeys has a superior biochemical composition when compared to the seminal plasma of the stallions in some components. Thus, the objective of this work will be to add autologous, homologous and heterologous plasma to sensitive and resistant cryopreservation animals and to evaluate the sperm viability of the cooled semen at 5 ° C for 24 hours. With this, it is expected that the addition of 20% of the heterologous plasma may bring improvements in the sperm viability of the refrigerated semen of sensitive stallions.