Evaluation of the UL111A transcripts expression in HCMV latent infected cells and determination of immunologic properties of the vIL10 proteins in dendritic cells

• Grant number: 18/19936-0
• Support Opportunities: Scholarships abroad - Research
• Start date: May 15, 2019
• End date: March 23, 2020
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Maria Cristina Carlan da Silva
• Grantee: Maria Cristina Carlan da Silva
• Host Investigator: John Henry Sinclair
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Institution abroad: University of Cambridge, England

Abstract

The Human Cytomegalovirus (HCMV) is a ubiquitous agent highly prevalent worldwide. HCMV is able to persist in a latent stage in the host for life and can cause serious diseases in immunosuppressed and newborns. It is known that during latency various cellular proteins are expressed in order to avoid immune survailance. However the molecular mechanisms of establishment of latency and reactivation are not well known. HCMV has an homologue of the human interleukin 10, denominated vIL-10. Two vIl-10 isoforms denominated cmvIl-10 and LAcmvIl-10 are expressed during litic replication and latency. LAcmvIL-10 has functions of immune evasion during latency. In addition , there are five transcrits (vIL-10 C to G) produced during litic infection, reported in a single study. There are no studies regarding expression of these transscripts during latent infection, especially in CD14 + and CD34+ cells which are the ideal models used for studies of HCMV latency. Besides, little is known about the immunologic properties of these isoforms (vIL-10 C to G). The goals of this project are to investigate the expression of the vIL10 transcrits during HCMV latent infection and to analyze the immunologic properties of the isofroms A, B, C, D, E, F and G in dendritic cells.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

POOLE, EMMA; NEVES, TAINAN CERQUEIRA; OLIVEIRA, MARTHA TRINDADE; SINCLAIR, JOHN; DA SILVA, MARIA CRISTINA CARLAN. Human Cytomegalovirus Interleukin 10 Homologs: Facing the Immune System. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, JUN 9 2020. (18/19936-0)

POOLE, EMMA; SILVA, MARIA CRISTINA CARLAN DA; HUANG, CHRIS; PERERA, MARIANNE; JACKSON, SARAH; GROVES, IAN J.; WILLS, MARK; RANA, AMER; SINCLAIR, JOHN. A BMPR2/YY1 Signaling Axis Is Required for Human Cytomegalovirus Latency in Undifferentiated Myeloid Cells. MBIO, v. 12, n. 3, MAY-JUN 2021. (18/19936-0)