MRM-profiling: a new tool for detecting biomarkers

• Grant number: 19/03385-7
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: May 21, 2019
• End date: October 22, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Paulo Clairmont Feitosa de Lima Gomes
• Grantee: César Augusto Marasco Júnior
• Supervisor: Robert G. Cooks
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: Purdue University, United States
• Associated to the scholarship: 18/11700-7 - Determination of pharmaceuticals in wastewater, BP.MS

Abstract

After ingestion and metabolism, chemicals in the environment can be eliminated from the body unchanged and/or converted into metabolites, in urine, feces, sweat or even tears. Currently about more than 300 environmental chemicals or their metabolites are measured in human samples (e.g. urine, blood, serum, breast milk). Nevertheless, many of these substances or even their metabolites remain in the organism for long periods and have potential value as biomarkers. As such they can be examined in biological samples or when excreted into the environment, in environmental samples. In this research project, we intend to detect potential biomarkers of environmental exposure. The chosen substances are caffeine, cotinine, aflatoxin and N,N-diethyl-meta-toluamide (DEET), in biological and environmental matrices, such as blood, urine and wastewater, respectively, using MRM-profiling as an exploratory technique and to evaluate the potential of these substances as biomarkers. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JUNIOR, CESAR A. MARASCO; EDWARDS, MADISON E.; LAMARCA, RAFAELA S.; SOBREIRA, TIAGO J. P.; CATERINO, JEFFREY M.; HAINS, DAVID S.; SCHWADERER, ANDREW L.; GOMES, PAULO CLAIRMONT F. DE LIMA; FERREIRA, CHRISTINA R.. Suspect screening of exogenous compounds using multiple reaction screening (MRM) profiling in human urine samples. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1201, p. 7-pg., 2022-06-30. (18/11700-7, 19/03385-7, 18/22393-8, 14/50945-4)

EDWARDS, MADISON E.; MARASCO, CESAR A.; SCHOCK, TRACEY B.; SOBREIRA, TIAGO J. P.; FERREIRA, CHRISTINA R.; COOKS, R. GRAHAM. Exploratory analysis using MRM profiling mass spectrometry of a candidate metabolomics sample for testing system suitability. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 468, OCT 2021. (18/11700-7, 19/03385-7)