Functional evaluation of the mitochondrial uncoupling proteins from Arabidopsis thaliana using insertion mutants

• Grant number: 18/19021-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2019
• End date: September 30, 2021
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Ivan de Godoy Maia
• Grantee: Rômulo Pedro Macêdo Lima
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Mitochondrial uncoupling proteins (UCP) are specialized proteins involved in mitochondrial transport, which are able to dissipate the proton electrochemical gradient generated by the respiration in the form of heat. These proteins are part of the mitochondrial anionic carrier family and they play a key role in the maintenance of mitochondrial function, being dependent on fatty acids and sensitive to purine nucleotides. In plants, their importance as a component of cell tolerance to oxidative stress has been shown in previous studies performed both in vitro and in vivo. Three genes encoding UCP have been identified in Arabidopsis thaliana (named AtUCP1-3), but to date, relevant functional data was only obtained for AtUCP1. Therefore, the role and physiological relevance of the other isoforms stills obscure. This study intends to take the phenotypic evaluation and functional redundancy of double mutants in different gene combinations (atucp1/atucp2, atucp1/atucp3 e atucp2/atucp3). For that, double mutants will be identified and phenotypically analyzed. In parallel, the overall expression and metabolite profiles of these double mutants, as well as transcriptome sequencing of atucp2 and atucp3 single mutants using RNA-Seq will be determined (under normal conditions and under stress) and compared with the data obtained for the previously characterized atucp1 mutant. The data to be generated will provide crucial information to improve our understanding of the respective roles of these proteins in plants aiming future applications.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARRETO, PEDRO; ARCURI, MARIANA L. C.; LIMA, ROMULO PEDRO MACEDO; MARINO, CELSO LUIS; MAIA, IVAN G.. omprehensive In Silico Analysis and Transcriptional Profiles Highlight the Importance of Mitochondrial Dicarboxylate Carriers (DICs) on Hypoxia Response in Both Arabidopsis thaliana and Eucalyptus grandi. PLANTS-BASEL, v. 11, n. 2, JAN 2022. (18/19021-1, 17/25139-2)

CAMPOS ARCURI, MARIANA DE LARA; NUNES-LAITZ, ALESSANDRA VASCONCELLOS; MACEDO LIMA, ROMULO PEDRO; BARRETO, PEDRO; MARINHO, ANDRESSA NAGATANI; ARRUDA, PAULO; MAIA, IVAN G.. Knockdown of Mitochondrial Uncoupling Proteins 1 and 2 (AtUCP1 and 2) in Arabidopsis thaliana Impacts Vegetative Development and Fertility. Plant and Cell Physiology, v. 62, n. 10, p. 1630-1644, OCT 2021. (17/25139-2, 18/19021-1)

LIMA, ROMULO PEDRO MACEDO; NUNES-LAITZ, ALESSANDRA VASCONCELLOS; ARCURI, MARIANA DE LARA CAMPOS; CAMPOS, FELIPE GIROTTO; JOCA, THAIS ARRUDA COSTA; MONTEIRO, GEAN CHARLES; KUSHIMA, HELIO; LIMA, GIUSEPPINA PACE PEREIRA; ALMEIDA, LUIZ FERNANDO ROLIM DE; BARRETO, PEDRO; et al. The double knockdown of the mitochondrial uncoupling protein isoforms reveals partial redundant roles during Arabidopsis thaliana vegetative and reproductive development. Plant Science, v. 322, p. 13-pg., 2022-07-12. (18/19021-1, 17/25139-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LIMA, Rômulo Pedro Macêdo. Functional evaluation of mitochondrial uncoupling proteins in Arabidopsis thaliana using insertion mutants. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.