In the last ten years, our laboratory has been studying the mitochondrial bioenergetics of Aspergillus fumigatus. We have shown the presence of classical components of the respiratory chain (or via of cytochrome c) in this microorganism. In addition, this fungus presents alternative components as alternative NADH dehydrogenase, an alternative oxidase and uncoupling protein-like. In addition to the input of NADH to the respiratory chain via classical and alternative NADH dehydrogenase, studies have identified and characterized two isoforms of mitochondrial protein carrier NAD+ in Arabidopsis thaliana (Ndt1p and Ndt2p, respectively). In previous studies in our laboratory, we observed that in mitochondria of the Paracoccidioides brasiliensis, the mitochondrial membrane potential could be induced by NAD+ and dissipated by uncoupler FCCP, suggesting the presence of a carrier NAD+, as described in S. cerevisiae. Other experiments were performed with mitochondria from A. fumigatus and also suggested the presence of this carrier in this organism. Using bioinformatic approaches, we found a sequence in the genome of A. fumigatus, which has 32% identity with the nicotinamide dinucleotide transporter gene (ndt1p) of S. cerevisiae. The objective of this project is cloning, sequence and heterologous expression of the ndt1p-like gene from A. fumigatus in the S.cerevisiae mutant strain (deltandt1deltandt2).
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