Heterologous expression of mitochondrial nicotinamide adenine dinucleotide transporter (Ndt1) from Aspergillus fumigatus rescues impaired growth in Delta ndt1 Delta ndt2 Saccharomyces cerevisiae strain

Author(s)

de Lima Balico, Lais de Lourdes ^[1] ; Santos, Emerson de Souza ^[1] ; Suzuki-Hatano, Silveli ^[1] ; Sousa, Lucas Oliveira ^[1] ; Caleiro Seixas Azzolini, Ana Elisa ^[1] ; Lucisano-Valim, Yara Maria ^[1] ; Dinamarco, Taisa Magnani ^[1] ; Kannen, Vinicius ^[1] ; Uyemura, Sergio Akira ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Bioenergetics and Biomembranes; v. 49, n. 6, p. 423-435, DEC 2017.
• Web of Science Citations: 0

Abstract

Our understanding of nicotinamide adenine dinucleotide mitochondrial transporter 1 (Ndt1A) in Aspergillus fumigatus remains poor. Thus, we investigated whether Ndt1A could alter fungi survival. To this end, we engineered the expression of an Ndt1A-encoding region in a Delta ndt1 Delta ndt2 yeast strain. The resulting cloned Ndt1A protein promoted the mitochondrial uptake of nicotinamide adenine dinucleotide (NAD(+)), generating a large mitochondrial membrane potential. The NAD(+) carrier utilized the electrochemical proton gradient to drive NAD(+) entrance into mitochondria when the mitochondrial membrane potential was sustained by succinate. Its uptake has no impact on oxidative stress, and Ndt1A expression improved growth and survival of the Delta ndt1 Delta ndt2 Saccharomyces cerevisiae strain. (AU)

• FAPESP's process: 12/15861-9 - Biochemical and molecular studies of the mitochondrial components of Aspergillus fumigatus: Cloning and heterologous expression of NAD/NADH carrier.
• Grantee: Laís de Lourdes de Lima Bálico
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 13/20031-8 - Mitochondrial componentes of the Aspergillus fumigatus: bioenergetics and molecular studies of the nicotinamide dinucleotides carrier
• Grantee: Sergio Akira Uyemura
• Support type: Regular Research Grants