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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Heterologous expression of mitochondrial nicotinamide adenine dinucleotide transporter (Ndt1) from Aspergillus fumigatus rescues impaired growth in Delta ndt1 Delta ndt2 Saccharomyces cerevisiae strain

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de Lima Balico, Lais de Lourdes [1] ; Santos, Emerson de Souza [1] ; Suzuki-Hatano, Silveli [1] ; Sousa, Lucas Oliveira [1] ; Caleiro Seixas Azzolini, Ana Elisa [1] ; Lucisano-Valim, Yara Maria [1] ; Dinamarco, Taisa Magnani [1] ; Kannen, Vinicius [1] ; Uyemura, Sergio Akira [1]
Total Authors: 9
[1] Univ Sao Paulo, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Bioenergetics and Biomembranes; v. 49, n. 6, p. 423-435, DEC 2017.
Web of Science Citations: 0

Our understanding of nicotinamide adenine dinucleotide mitochondrial transporter 1 (Ndt1A) in Aspergillus fumigatus remains poor. Thus, we investigated whether Ndt1A could alter fungi survival. To this end, we engineered the expression of an Ndt1A-encoding region in a Delta ndt1 Delta ndt2 yeast strain. The resulting cloned Ndt1A protein promoted the mitochondrial uptake of nicotinamide adenine dinucleotide (NAD(+)), generating a large mitochondrial membrane potential. The NAD(+) carrier utilized the electrochemical proton gradient to drive NAD(+) entrance into mitochondria when the mitochondrial membrane potential was sustained by succinate. Its uptake has no impact on oxidative stress, and Ndt1A expression improved growth and survival of the Delta ndt1 Delta ndt2 Saccharomyces cerevisiae strain. (AU)

FAPESP's process: 12/15861-9 - Biochemical and molecular studies of the mitochondrial components of Aspergillus fumigatus: Cloning and heterologous expression of NAD/NADH carrier.
Grantee:Laís de Lourdes de Lima Bálico
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/20031-8 - Mitochondrial componentes of the Aspergillus fumigatus: bioenergetics and molecular studies of the nicotinamide dinucleotides carrier
Grantee:Sergio Akira Uyemura
Support type: Regular Research Grants