Comparison of two commercial kits for RNA extraction from formalin fixed and paraffin embedded equine experimental wound samples

• Grant number: 18/22295-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2019
• End date: September 30, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Flávia de Almeida Lucas
• Grantee: Gabriel Freitas Urzedo
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The objective of this study is to compare two commercial kits indicated for extracting RNA with quality for transcriptome and tissue miRNA profiles of fixed and formalin and paraffin embedded samples (FEPP). FEPP samples obtained in a previous research will be used, where biopsies of the cutaneous wounds performed on four horses were collected on day three and 21 after the surgical preparation of the same. Two commercial kits indicated for RNA extraction from commercially available FEPP samples will be used to compare the quality of extracted RNA.