Detection of EcPV-3 and EcPV-4 in paraffin-embedded aural plaque samples using PCR
| Grant number: | 18/22295-6 |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| Effective date (Start): | April 01, 2019 |
| Effective date (End): | September 30, 2020 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery |
| Principal Investigator: | Flávia de Almeida Lucas |
| Grantee: | Gabriel Freitas Urzedo |
| Home Institution: | Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil |
Abstract The objective of this study is to compare two commercial kits indicated for extracting RNA with quality for transcriptome and tissue miRNA profiles of fixed and formalin and paraffin embedded samples (FEPP). FEPP samples obtained in a previous research will be used, where biopsies of the cutaneous wounds performed on four horses were collected on day three and 21 after the surgical preparation of the same. Two commercial kits indicated for RNA extraction from commercially available FEPP samples will be used to compare the quality of extracted RNA. | |