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Comparison of two commercial kits for RNA extraction from formalin fixed and paraffin embedded equine experimental wound samples

Grant number: 18/22295-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2019
Effective date (End): September 30, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Flávia de Almeida Lucas
Grantee:Gabriel Freitas Urzedo
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The objective of this study is to compare two commercial kits indicated for extracting RNA with quality for transcriptome and tissue miRNA profiles of fixed and formalin and paraffin embedded samples (FEPP). FEPP samples obtained in a previous research will be used, where biopsies of the cutaneous wounds performed on four horses were collected on day three and 21 after the surgical preparation of the same. Two commercial kits indicated for RNA extraction from commercially available FEPP samples will be used to compare the quality of extracted RNA.