Effect of in vitro administration of grape seed extract in the functional activity of MC3T3-E1 osteoblastic cell line

• Grant number: 19/05827-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2019
• End date: May 31, 2020
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Karina Fittipaldi Bombonato Prado
• Grantee: Maria Carolina Coelho
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Osteoporosis is a major concern in public health, mainly affecting elder people and women after menopause. Drug therapy for this disease shows positive effects on bone metabolism but may promote several side effects. Thus, an alternative would be the use of natural substances like grape seed extract (GSE), which has a great antioxidant potential for containing proanthocianidines (PACs). The aim of this study is to perform in vitro studies to evaluate the effect of GSE in the functional activity of osteoblastic cells from MC3T3-E1 cell line. Cells will be cultured in flasks in osteogenic medium until subconfluence and seeded at a concentration of 2 x 104 cells per well in culture plates (n = 5). Experimental groups will be divided in control cells (C), and treated with 1mgl/mL GSE. After the experimental periods, the following parameters will be evaluated: adhesion and cell proliferation, alkaline phosphatase in situ detection, total protein content, quantification of mineralized nodules and immunolocalization of alkaline phosphatase and bone sialoprotein. The results will be analyzed by statistical programs consistent with significance p d 0,05, with the goal of finding statistically significant data showing the beneficial effects of GSE in osteoblast activity.