Osteoporosis is a major concern in public health, mainly affecting elder people and women after menopause. Drug therapy for this disease shows positive effects on bone metabolism but may promote several side effects. Thus, an alternative would be the use of natural substances like grape seed extract (GSE), which has a great antioxidant potential for containing proanthocianidines (PACs). The aim of this study is to perform in vitro studies to evaluate the effect of GSE in the functional activity of osteoblastic cells from MC3T3-E1 cell line. Cells will be cultured in flasks in osteogenic medium until subconfluence and seeded at a concentration of 2 x 104 cells per well in culture plates (n = 5). Experimental groups will be divided in control cells (C), and treated with 1mgl/mL GSE. After the experimental periods, the following parameters will be evaluated: adhesion and cell proliferation, alkaline phosphatase in situ detection, total protein content, quantification of mineralized nodules and immunolocalization of alkaline phosphatase and bone sialoprotein. The results will be analyzed by statistical programs consistent with significance p d 0,05, with the goal of finding statistically significant data showing the beneficial effects of GSE in osteoblast activity.
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