Study of the best trajectory and caliber of needles used in the processes of renal biopsy in cats

• Grant number: 19/00411-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2019
• End date: July 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Leandro Zuccolotto Crivellenti
• Grantee: Maria Eduarda Raffaini de Oliveira Cunha
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Renal biopsy is an effective procedure to diagnose kidney disease and to enable the nephrologist to better target when adopting a therapeutic approach. Due to its importance, the present study tries to analyze the best trajectory of the needles, as well as their diameter when performing the biopsy procedure in kidneys from domestic felines, aiming to obtain techniques that result in quality samples with reduced risk of complications. The objective of this study was to analyze the targeting and diameter of the needles, as well as the quality of the renal samples and possible repercussions for the biopsied animals. Five cat carcasses will be used. Of these, four will be used for renal biopsy with Tru cut® needle 14, 16 and 18 in the cranial and caudal poles and in the lateral portion of the kidneys (Tru-cuts Group). A cat will be randomly selected for kidney section for instrumentation-assisted virtual instrumentation analysis of the regions of the poles and the middle portion for global counting of glomeruli (virtual biopsy group), which will simulate samples obtained by biopsy, thus determining the largest quantity of glomeruli and also the best caliber of the needle, besides analyzing the possible presence of medullary tissue and medium caliber arteries that could lead to complications if collected from a patient. Samples will be processed for routine inclusion in paraffin and submitted to standard staining (hematoxylin and eosin and Schiff's periodic acid reaction) for evaluation of renal histoarchitecture using quantitative microscopy (morphometric and stereological analyzes). After obtaining the groups, they will be compared in order to establish which of the needle directions is the best and free of evidence that could cause complications in vivo.