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Effect of 3D Scaffold on coagulum features and osteogenesis: in vitro and ex vivo study

Grant number: 18/26435-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2019
Effective date (End): August 31, 2020
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Daniela Bazan Palioto Bulle
Grantee:Hilary Ignes Palma Caetano
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

In oral reconstruction, biomaterials are commonly used as scaffolds that enable restoration of bone integrity. On the other hand, three-dimensional (3D) scaffolds have been understood as being the most interesting, since they provide a homogeneous cellular distribution and, consequently, high osteoconductivity. Many materials have been developed for 3D scaffolds preparation. Among them, hydrogel is an ideal candidate in this regard. In bone regeneration process, blood is the first component to come into contact with the biomaterials used. Therefore, this study aims to see the effects of 3D hydrogel scaffolds (SCA) on blood clot formation and, additionally, osteogenesis. First, MTT assay will test in vitro cell viability of rat-derived osteoblastic (OSB) cells (UMR-106 lineage) on SCA. The physiological clot (PhC) will then be developed in an animal model (rat) on SCA, characterizing the ex vivo assay. For this, we will use Hannover male rat, aged 12 weeks. Experimental protocol will be modified from Monroe et al. (2012), with PhC being created in rat calvarial defects. Thus, PhC phenotyping will be characterized by scanning electron microscopy, making it possible to verify all the major blood clot components. In another step, the OSB will be grown on the substrate (SCA and PhC), aiming to observe its effect on formation and accumulation of calcium in OSB by Alizarin red stain assay. These findings will be promising to observe both in vitro and ex vivo effects of this biomaterial on blood clot formation and osteogenesis.