SYNERGISTIC ACTIVITY OF BLUE LIGHT IN COMBINATION WITH CHEMOTHERAPEUTIC ANTIMICROBIAL AGAINST DRUG-RESISTANT PATHOGENS

• Grant number: 19/10851-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2019
• End date: September 30, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Fabio Celidonio Pogliani
• Grantee: Carolina dos Anjos
• Supervisor: Tianhong Dai
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Massachusetts General Hospital, United States
• Associated to the scholarship: 16/25095-2 - BACTERIAL PHOTOINACTIVATION OF MASTITIS PATHOGENS BY BLUE LIGHT: MECHANISMS OF ACTION AND CELL SECURITY - PRE-CLINICAL STUDY, BP.DR

Abstract

Antimicrobial resistance emerges as one of the principal public health problems of the century. The pharmaceutical industry faces major challenges, and the development of new drug does not accompany antimicrobial resistance rises. In this context, there is an urgent need for studies addressing the combination of existing drugs in conjunction with new antimicrobial alternatives. We propose to investigate the synergistic activity of bacterial photoinactivation by blue light in combination with chemotherapeutic antimicrobial agents used in the clinical routine. This study will be part of the PhD project titled "Bacterial photoinactivation of mastitis pathogens by blue light: mechanisms of action and cell security - pre-clinical study " funded by FAPESP (PROCESS #2016/25095-2). Will be conducted under the supervision of Prof. Tianhong Dai, PhD, at the Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA. Drug-resistance bacteria belonging to the ESKAPE group (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanni, Pseudomonas aeruginosa, and Escherichia coli) will be used. The combination will be performed as recommended by CLSI to minimal inhibitory concentration. The interpretation of these results will be complementary to our studies and can indirectly elucidate the structural damage targets promoted by the blue light.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BESEGATO, JOAO FELIPE; GOBBO DE MELO, PRISCILA BORGES; TAMAE, PATRICIA ERIKO; APARECIDA RAIMUNDO ALVES, ANA PAULA; RONDON, LUIS FELIPE; LEANSE, LEON G.; DOS ANJOS, CAROLINA; CASARIN, HEITOR HUSSNI; CHINELATTI, MICHELLE ALEXANDRA; FARIA, GISELE; et al. ow can biophotonics help dentistry to avoid or minimize cross infection by SARS-CoV-2. Photodiagnosis and Photodynamic Therapy, v. 37, MAR 2022. (13/07276-1, 19/10851-4)

LEANSE, LEON G.; DOS ANJOS, CAROLINA; WANG, YING; MURRAY, CLINTON K.; HOOPER, DAVID C.; DAI, TIANHONG. Effective Treatment of Cutaneous Mold Infections by Antimicrobial Blue Light That Is Potentiated by Quinine. Journal of Infectious Diseases, v. 224, n. 6, p. 1069-1076, SEP 15 2021. (19/10851-4)

DOS ANJOS, CAROLINA; SABINO, CAETANO P.; SELLERA, FABIO P.; ESPOSITO, FERNANDA; POGLIANI, FABIO C.; LINCOPAN, NILTON. Hypervirulent and hypermucoviscous strains of Klebsiella pneumoniae challenged by antimicrobial strategies using visible light. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, v. 56, n. 1, JUL 2020. (19/10851-4, 16/25095-2, 19/15578-4, 17/22406-0)

DOS ANJOS, CAROLINA; LEANSE, LEON G.; RIBEIRO, MARTHA S.; SELLERA, FABIO P.; DROPA, MILENA; ARANA-CHAVEZ, VICTOR E.; LINCOPAN, NILTON; BAPTISTA, MAURICIO S.; POGLIANI, FABIO C.; DAI, TIANHONG; et al. New Insights into the Bacterial Targets of Antimicrobial Blue Light. MICROBIOLOGY SPECTRUM, v. 11, n. 2, p. 13-pg., 2023-02-21. (16/25095-2, 19/10851-4)

DOS ANJOS, CAROLINA; SELLERA, FABIO P.; RIBEIRO, MARTHA S.; BAPTISTA, MAURICIO S.; POGLIANI, FABIO C.; LINCOPAN, NILTON; SABINO, CAETANO P.. Antimicrobial blue light and photodynamic therapy inhibit clinically relevant 8-lactamases with extended-spectrum (ESBL) and carbapenemase activity. Photodiagnosis and Photodynamic Therapy, v. 32, DEC 2020. (16/25095-2, 19/10851-4, 17/22406-0)

LEANSE, LEON G.; DOS ANJOS, CAROLINA; BESEGATO, JOAO FELIPE; DAI, TIANHONG; RASTELLI, ALESSANDRA N. S.. Shedding UVC light on Covid-19 to protect dentistry staff and patients. LASER PHYSICS LETTERS, v. 18, n. 8, AUG 2021. (13/07276-1, 14/50857-8, 19/10851-4)

LEANSE, LEON G.; DOS ANJOS, CAROLINA; MUSHTAQ, SANA; DAI, TIANHONG. Antimicrobial blue light: A 'Magic Bullet' for the 21st century and beyond?. Advanced Drug Delivery Reviews, v. 180, p. 27-pg., 2022-01-01. (19/10851-4)

SABINO, CAETANO P.; RIBEIRO, MARTHA S.; WAINWRIGHT, MARK; DOS ANJOS, CAROLINA; SELLERA, FABIO P.; DROPA, MILENA; NUNES, NATHALIA B.; BRANCINI, GUILHERME T. P.; BRAGA, GILBERTO U. L.; ARANA-CHAVEZ, VICTOR E.; et al. The Biochemical Mechanisms of Antimicrobial Photodynamic Therapy(1). Photochemistry and Photobiology, v. N/A, p. 9-pg., 2022-08-12. (16/25095-2, 17/22406-0, 16/08593-9, 13/07937-8, 19/10851-4)