Multivariate calibration applied to the monitoring of the enzymatic synthesis of galactooligosaccharide

• Grant number: 18/26443-0
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2019
• Effective date (End): July 31, 2020
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Principal researcher: Marcelo Perencin de Arruda Ribeiro
• Grantee: Raissa Guidolin Marcato
• Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

Cheese whey (or its permeate), commonly discarded by small dairy producers, may have an alternative destination such as the enzymatic synthesis of galactooligosaccharides (GOS) using b-galactosidase. GOS has high added value and the enzyme b-galactosidase is considered a GRAS enzyme, and therefore without the need to separate it from the product. However, there are several parameters that affect the yield of lactose in GOS besides the high cost of the enzyme, which can make the investment risky in this process. As the selectivity between synthesis and hydrolysis increases with the lactose concentration, it is important to maintain high lactose concentration through fed batch so the yield is increased. The monitoring of reactor status is important to know the feed rate of lactose and to feed enzyme solution, since this tends to inactivate during the synthesis.