Cheese whey (or its permeate), commonly discarded by small dairy producers, may have an alternative destination such as the enzymatic synthesis of galactooligosaccharides (GOS) using b-galactosidase. GOS has high added value and the enzyme b-galactosidase is considered a GRAS enzyme, and therefore without the need to separate it from the product. However, there are several parameters that affect the yield of lactose in GOS besides the high cost of the enzyme, which can make the investment risky in this process. As the selectivity between synthesis and hydrolysis increases with the lactose concentration, it is important to maintain high lactose concentration through fed batch so the yield is increased. The monitoring of reactor status is important to know the feed rate of lactose and to feed enzyme solution, since this tends to inactivate during the synthesis.
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