Silencing of B1R and B2R kinin receptors in the BV-2 microglial cell line as a model of neuroinflammation response

• Grant number: 19/03494-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2019
• End date: August 31, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Alexander Henning Ulrich
• Grantee: Jean Bezerra Silva
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/07366-4 - Purine and kinin receptors as targets of study and therapeutic interventions in neurological diseases, AP.TEM

Abstract

Inflammatory processes are commonly observed in different pathological conditions. When they occur in the central nervous system, these processes are mostly mediated by microglia cells. The functional role of microglia depends on their polarization and activation profile in order to guarantee homeostatic balance and defense of the nervous system. Among the pathways and mediating molecules of neuroinflammatory responses, the kallikrein-kinins system has been prominent due to its importance for processes such as vasodilation, increased vascular permeability, eicosanoid synthesis, the production and release of cytokines and the Wnt/GSK-3² pathway modulation. The present project aims to evaluate the involvement of kinins in neuroinflammatory processes in vitro; more specifically, the role of kinin B1 and B2 receptors in the microglial polarization and in the modulation of the Wnt/GSK-3B pathway in the BV-2 murine microglial cell line will be investigated.