Microbiome of oral fluid of pigs and its relationship with fecal microbioma

• Grant number: 19/19473-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2019
• End date: October 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Vera Letticie de Azevedo Ruiz
• Grantee: Paloma Alonso Madureira
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

Several research groups studied swine microbiome of different samples, generally finding high frequency of Bacteriodetes e Firmicutes phyla, but no group studied the microbial composition of oral fluid until the present moment. The collection of oral fluid is presented as an alternative for the search for antibodies and pathogens, with sensitivity similar to traditional methods and smaller impacts to animal welfare at the time of collection. Thus, the objective of this project will be to determine the microbioma of oral fluid of pigs and their relationships with the fecal microbioma composition and to verify the relationships between these compositions. We expect that the sum of these results could support the use of oral fluid as a sample of choice for monitoring and sanitary surveys, favoring the collection of samples for the technician and reducing the impact on animal welfare, maintaining the diagnostic specificity and sensitivity.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, VINICIUS BUIATTE DE ANDRADE; CARVALHO, ENEAS; MADUREIRA, PALOMA ALONSO; MARINO, ELIZANGELA DOMENIS; VAZ, ANDREIA CRISTINA NAKASHIMA; VIDAL, ANA MARIA CENTOLA; RUIZ, VERA LETTICIE DE AZEVEDO. First isolation and whole-genome sequencing of a Shewanella algae strain from a swine farm in Brazil. BMC Microbiology, v. 20, n. 1, DEC 24 2020. (19/19473-2, 17/22184-7)

BUIATTE, VINICIUS; FONSECA, ANA; MADUREIRA, PALOMA ALONSO; VAZ, ANDREIA CRISTINA NAKASHIMA; TIZIOTO, POLYANA CRISTINE; VIDAL, ANA MARIA CENTOLA; GANDA, ERIKA; RUIZ, VERA LETTICIE DE AZEVEDO. A comparative study of the bacterial diversity and composition of nursery piglets' oral fluid, feces, and housing environment. SCIENTIFIC REPORTS, v. 14, n. 1, p. 13-pg., 2024-02-19. (19/19473-2, 17/22184-7)