Evaluation of microbial reduction of different NiTi instruments in oval root canals infected by Enterococcus faecalis biofilm: an in vitro study

• Grant number: 19/24892-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2020
• End date: January 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Rogerio de Castilho Jacinto
• Grantee: Yana da Silva Maia
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The biomechanical preparation while modeling the dentin walls, giving optimal shape, promotes the cleaning of the channels contaminated by bacterial biofilm. During instrumentation, debris and bacteria can be extruded into the periapical region, causing or exacerbating a local inflammatory process. The anatomical complexities of the root canals influence the instrumentation, making the instruments unable to contact all the canal walls. To address this failure in instrumentation and disinfection, several systems have been developed. This study aims to compare intracanal bacterial reduction using XP-3D Shape (Brasseler USA®), TRUShape (Dentsply) and Reciproc (VDW) files. The research will be performed on human teeth, 40 extracted lower central incisors, will undergo an initial radiographic analysis, then the samples will be contaminated with Enterococcus faecalis strains and instrumented from the manufacturer's recommendations for use. Microbiological analyzes of bacterial reduction will be done by bacterial culture. Data will be tabulated and statistically analyzed using the SigmaPlot 12.0 program (Systat Software Inc., San Jose, USA) at a significance level of 5%. (AU)